Best-in-class RNA ISH for your automated system of choice

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems and Roche Tissue Diagnostics to deliver RNAscope® ISH automated assays.

RNAscope® 2.5 LS Reagent Kits and RNAscope® LS Duplex Reagent Kits are offered for a fully automated walk-away solution on the Leica BOND RX System by Leica Biosystems automating the Permeabilize, Hybridize and Amplify steps of the RNAscope® Workflow.

RNAscope® 2.5 VS Reagent Kits are offered for an automated walk-away solution on the DISCOVERY ULTRA and DISCOVERY XT automated tissue staining systems by Roche Tissue Diagnostics automating the Hybridize and Amplify steps of the RNAscope® Workflow. Most recently the RNAscope® VS Universal Assays offered exclusively for the DISCOVERY ULTRA allows for additional capability such as ISH-IHC or IF with Roche Tissue Diagnostic’s mRNA Universal protocol

RNAscope® Automated LS Assay

RNAscope® Automated VS Assay

RNAscope® In Situ Hybridization Assay Workflow

  • Step 1:

    Permeabilize

    Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.

  • Step 2:

    Hybridize

    Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.

  • Step 3:

    Amplify

    RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.

  • Step 4:

    Visualize

    Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.

  • Step 5:

    Quantify

    Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.

Automated
Automated

Which RNAscope® Automated Assay is right for me?

Assays & Features

RNAscope® 2.5 Leica Assay Assay - BROWN

RNAscope® 2.5 Leica Assay - RED

RNAscope® 2.5 LS Duplex Assay

RNAscope® 2.5 Ventana Assay - BROWN

RNAscope® 2.5 Ventana Assay - RED

Automated System

Leica BOND RX System

Leica BOND RX System

Leica BOND RX System

Ventana DISCOVERY ULTRA Ventana DISCOVERY XT

Ventana DISCOVERY ULTRA Ventana DISCOVERY XT

Assay Type

Chromogenic

Chromogenic

Chromogenic

Chromogenic

Chromogenic

Dye Used

Diaminobenzidine (DAB)

Fast Red

Diaminobenzidine (DAB) & Fast Red

Diaminobenzidine (DAB)

Fast Red

Multiplexing

Singleplex

Singleplex

Duplex

Singleplex

Singleplex

Key Benefit

Robust, sensitive, permanent stain Most widely used RNAscope assay

Provides bright color stains with high contrast to background

Simultaneous in situ detection of two RNA species

Robust, sensitive, permanent stain Most widely used RNAscope assay

Provides bright color stains with high contrast to background

Ideal for

High throughput Routine applications

Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers

Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor)

High throughput Routine applications

Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers

Novel Gene or Unkown Expression

+++++

+++++

+++++

+++++

+++++

Archival Specimens

+++++

++

++

+++++

++

Microscope Imaging System

Standard bright field

Standard bright field

Standard bright field

Standard bright field

Standard bright field

Sample Type

FFPE

FFPE

FFPE

FFPE

FFPE

Quantitation Method

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

# of tissue sections per kit

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

X
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For general information: Leon Li lli@acdbio.com
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