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Best-in-class RNA ISH for your automated system of choice

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems to deliver RNAscope® ISH automated assays.

RNAscope® 2.5 LS Reagent Kits and RNAscope® LS Duplex Reagent Kits are offered for a fully automated walk-away solution on the Leica BOND RX System by Leica Biosystems automating the Permeabilize, Hybridize and Amplify steps of the RNAscope® Workflow.

RNAscope® Automated LS Assay

Which Automated Assay is right for me?

RNAscope® 2.5 Leica Assay - BROWN

RNAscope® 2.5 Leica Assay - RED

RNAscope® 2.5 Leica Duplex Assay

RNAscope® Leica Multiplex Fluorescent Assay

BaseScope™ Leica Assay - RED

miRNAscope™ Leica Assay - RED

Automated System

Leica BOND RX System

Leica BOND RX System

Leica BOND RX System

Leica BOND RX System

Leica BOND RX System

Leica BOND RX System

Assay Type

Chromogenic

Chromogenic

Chromogenic

Fluorescent

Chromogenic

Chromogenic

Dye Used

Diaminobenzidine (DAB)

Fast Red

Diaminobenzidine (DAB) & Fast Red Option for Green & Fast Red

TSA-based Opal fluorophores

Fast Red

Fast Red

Multiplexing

Singleplex

Singleplex

Singleplex, Duplex

Single to Fourplex

Singleplex

Singleplex

Key Benefit

Robust, sensitive, permanent stain Most widely used RNAscope assay

Provides bright color stains with high contrast to background

Simultaneous in situ detection of two RNA species

Utilizes up to 4 different spectral channels providing high flexibility

Specific detection of splice variants/exon junctions and short targets

Robust and specific detection of small RNAs

Ideal for

High throughput Routine applications

Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers

Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor)

Co-localization studies of up to 4 gene simultaneously Experimental application requiring flexibility

Detection of splice variants and exon junctions, short insertions/deletions, short targets of complex & highly homologous gene families

Detection of small RNAs including ASOs, miRNAs, piRNAs, shRNAs, siRNAs, and tRNAs

Novel Gene or Unknown Expression

Compatible

Compatible

Compatible

Compatible

Compatible

Compatible

Archival Specimens

Compatible

Compatible

Compatible

Compatible

Compatible

Compatible

Microscope Imaging System

Standard bright field

Standard bright field

Standard bright field

Multispectral fluorescent imaging

Standard bright field

Standard bright field

Sample Type

FFPE
Fixed Frozen Tissues
Fresh Frozen Tissues
Cultured cells

FFPE
Fixed Frozen Tissues
Fresh Frozen Tissues
Cultured cells

FFPE
Fixed Frozen Tissues
Fresh Frozen Tissues
Cultured cells

FFPE Tissues(TMAs)
Fixed Frozen Tissues
Fresh Frozen Tissues
Cultured cells

FFPE
Fresh Frozen Tissues
Cultured cells

FFPE
Fixed Frozen
Fresh Frozen Tissues
Cultured cells

Quantitation Method

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

# of tissue sections per kit

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

Target Sequence length required

300-1000bp

300-1000bp

300-1000bp

300-1000bp

50-300bp

17-50bp

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Advanced Cell Diagnostics

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Advanced Cell Diagnostics China

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Beijing, China, Zip code: 100176

400-966-1796
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