Best-in-class RNA ISH for your automated system of choice
For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems to deliver RNAscope™ ISH automated assays.
RNAscope™ 2.5 LS Reagent Kits and RNAscope™ LS Duplex Reagent Kits are offered for a fully automated walk-away solution on the Leica BOND RX System by Leica Biosystems automating the Permeabilize, Hybridize and Amplify steps of the RNAscope Workflow.
RNAscope Automated LS Assay
Which Automated Assay is right for me?
RNAscope™ 2.5 Leica Assay - BROWN | RNAscope™ 2.5 Leica Assay - RED | RNAscope™ 2.5 Leica Duplex Assay | RNAscope™ Multiomic LS Assay | RNAscope™ Leica Multiplex Fluorescent Assay | BaseScope™ Leica Assay - RED | miRNAscope™ Leica Assay - RED | |
|---|---|---|---|---|---|---|---|
Automated System | Leica BOND RX System | Leica BOND RX System | Leica BOND RX System | Leica BOND RX System | Leica BOND RX System | Leica BOND RX System | Leica BOND RX System |
Assay Type | Chromogenic | Chromogenic | Chromogenic | Fluorescent | Fluorescent | Chromogenic | Chromogenic |
Dye Used | Diaminobenzidine (DAB) | Fast Red | Diaminobenzidine (DAB) & Fast Red Option for Green & Fast Red | TSA-based Opal fluorophores | TSA-based Opal fluorophores | Fast Red | Fast Red |
Multiplexing | Singleplex | Singleplex | Singleplex, Duplex | 1-plex - 6-plex | Single to Fourplex | Singleplex | Singleplex |
Key Benefit | Robust, sensitive, permanent stain Most widely used RNAscope assay | Provides bright color stains with high contrast to background | Simultaneous in situ detection of two RNA species | Enables same section protein and RNA detection. Up to 6-plex RNA, protein, or in combination. Available primary antibody panels for immediate cell phenotyping. Available secondary antibodies for use with user selectable primary antibodies. | Utilizes up to 4 different spectral channels providing high flexibility | Specific detection of splice variants/exon junctions and short targets | Robust and specific detection of small RNAs |
Ideal for | High throughput Routine applications | Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers | Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor) | Visualization of cell identification using protein markers and cell activation state with RNA. | Co-localization studies of up to 4 gene simultaneously Experimental application requiring flexibility | Detection of splice variants and exon junctions, short insertions/deletions, short targets of complex & highly homologous gene families | Detection of small RNAs including ASOs, miRNAs, piRNAs, shRNAs, siRNAs, and tRNAs |
Novel Gene or Unknown Expression | Compatible | Compatible | Compatible | Compatible | Compatible | Compatible | Compatible |
Archival Specimens | Compatible | Compatible | Compatible | Compatible | Compatible | Compatible | Compatible |
Microscope Imaging System | Standard bright field | Standard bright field | Standard bright field | Multispectral fluorescent imaging | Multispectral fluorescent imaging | Standard bright field | Standard bright field |
Sample Type | FFPE | FFPE | FFPE | FFPE Tissues(TMAs) | FFPE Tissues(TMAs) | FFPE | FFPE |
Quantitation Method | Visual inspection | Visual inspection | Visual inspection | Visual inspection | Visual inspection | Visual inspection | Visual inspection |
# of tissue sections per kit | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) |
Target Sequence length required | 300-1000bp | 300-1000bp | 300-1000bp | 300-1000bp | 300-1000bp | 50-300bp | 17-50bp |
