2018 Feb 20 - Tips, Tricks & Troubleshooting RNAscope™ and BaseScope™ Assays
Marie Lauigan
Technical Support Scientist
Watch Video:
Outline:

RNAscope Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH, with its proprietary probe design to amplify target-specific signals. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

Our recent advances in RNAscope technology include the development of the BaseScope assay, which enables detection of short RNA targets and exon junctions for the analysis of splice variants, circular RNA and gene fusions.

We will discuss the workflow of both RNAscope and BaseScope assays and share troubleshooting tips and tricks to obtain publication-quality RNA in situ results.

Who should attend:

This webinar will benefit:

  • New and experienced RNAscope & BaseScope technology users.
  • Users performing ISH combined with IHC
  • Traditional ISH users currently performing RNAscope ISH
Learning Objectives:
  • How RNAscope and BaseScope assays work
  • Workflow of RNAscope and BaseScope assays
  • What tools are needed for getting started
  • Tips and tricks for sample preparation & pretreatment
  • FAQs & Troubleshooting tips
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