HALO® image analysis platform

ACD has partnered with Indica Labs to offer quantitative RNAscope image analysis. This advanced digital pathology image analysis solution brings objective and accurate quantification to RNA in situ hybridization, enabling a new generation of applications based on single-cell analysis. With the ISHISH-IHCFISH, and FISH-IF modules of the HALO® image analysis platform, pathologists and researchers can perform quantitative gene expression analysis at single-cell resolution and interpret data in the histopathological context

RNAscope quantification with HALO provides:

  • Quantitative and reproducible RNAscope spot counting
  • Single-plex and multiplex ISH quantification
  • Brightfield and fluorescent ISH quantification
  • Cell-by-cell expression profiles
  • Automatic generation of H-scores and histograms
  • Rapid whole-slide analysis
  • Manual and automatic selection of distinct cell populations
  • Proximity Analysis, Nearest Neighbor Analysis, Infiltration Analysis, and Density Heatmap are available with the Spatial Analysis module


The HALO image analysis platform is compatible with all RNAscope Manual and Automated Assays:

  • RNAscope 2.5 HD Reagent Kit—BROWN
  • RNAscope 2.5 HD Reagent Kit—RED
  • RNAscope 2.5 LS Duplex Assay
  • RNAscope 2.5 LS Fluorescent Multiplex Assay
  • RNAscope 2.5  LSx Reagent Kit—BROWN
  • RNAscope 2.5 LSx Reagent Kit—RED
  • BaseScope LS Reagent Kit —RED
  • miRNAscope LS Reagent Kit Red
  • RNAscope VS Universal HRP
  • RNAscope VS Universal AP
  • RNAscope VS Duplex Assay
  • BaseScope VS Reagent Kit —RED
  • RNA-Protein Co-detection Ancillary Kit
  • DNAscope Duplex Assay


ACD offers image analysis as part of our Professional Assay Services (Learn more).


The HALO image analysis platform is also available for purchase and deployment within your organization. For more information contact info@indicalab.com.

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Advanced Cell Diagnostics

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