RNAscope® 2.5 HD Duplex Assay

The RNAscope® 2.5 HD Duplex Assay is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in FFPE samples mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH. RNAscope® probes in Channel 1 and Channel 2 are compatible with this assay which utilizes HRP-based Green and AP-based Fast Red chromogens to result in a detectable green and red signal respectively. The RNAscope® 2.5 HD Duplex Assay can be used for simultaneous visualization of two RNA targets while maintaining single cell resolution. The applications of this assay include study of gene co-regulation, profile gene expression in a specific cell type (identified using a cell specific marker) and map coexpression of two targets  within the same cellular context based on reliable chromogenic detection of the two transcripts.

Sample Images
RNA in situ expression of Hs-EGFR-no-X-Mm (green) and Mm-EGFR-no-X-Hs (red) in patient-derived xenograft sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of Hs-EGFR-no-X-Mm (green) and Mm-EGFR-no-X-Hs (red) in patient-derived xenograft sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of NRG1 (green) and ERBB3 (red) in human breast cancer FFPE tissue using RNAscope<sup>®</sup> 2.5 HD Duplex Assay

RNA in situ expression of NRG1 (green) and ERBB3 (red) in human breast cancer FFPE tissue using RNAscope® 2.5 HD Duplex Assay

RNA in situ expression of CD274 (green) and CD8A (red) in human lung cancer FFPE tissue using RNAscope<sup>®</sup> 2.5 HD Duplex Assay

RNA in situ expression of CD274 (green) and CD8A (red) in human lung cancer FFPE tissue using RNAscope® 2.5 HD Duplex Assay

RNA in situ expression of CD248 (green) and PDGFRB (red) in human colon cancer FFPE sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of CD248 (green) and PDGFRB (red) in human colon cancer FFPE sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of Hs-PECAM1-no-XMm (green) and Mm-PECAM1 (red) in patient-derived xenograft sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of Hs-PECAM1-no-XMm (green) and Mm-PECAM1 (red) in patient-derived xenograft sample using RNAscope 2.5 HD Duplex Assay

RNA in situ expression of CD274 (green) and CD8A (red) in human lung cancer FFPE tissue using RNAscope<sup>®</sup> 2.5 HD Duplex Assay

RNA in situ expression of CD274 (green) and CD8A (red) in human lung cancer FFPE tissue using RNAscope® 2.5 HD Duplex Assay

RNAscope® 2.5 HD Duplex  Reagent Kit  gives reliable results which are ideal for archiving due to the permanent staining visible under any standard bright field microscope. The RNAscope® 2.5 HD Duplex Reagent Kits are designed to provide more intense staining when low copy target gene expression is anticipated (1–20 copies per cell). The RNAscope® 2.5 HD Duplex reagent kits are compatible with the same RNAscope® probes used previously with RNAscope®2.0 2-plex assays.

For customers using RNAscope® 2.5 HD Duplex Manual Assay for the first time, ACD's Intro Packs  include species-specific control slides, control probes, the Reagent Kit, the ImmEdge hydrophobic barrier pen and the must-have accessory VectaMount™ Mounting Medium from Vecta Labs.  Be sure to order your target C1 and C2 probes separately.

The RNAscope® 2.5 HD Duplex Manual Assay Workflow Requires:

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