The RNAscope ® assay provides a powerful method to detect gene expression within the spatial and morphological context of the tissue. The proprietary “double Z” probe design in combination with advanced signal amplification techniques enables highly specific and sensitive detection of target RNA, which can be visualized as a dot, with each dot representing a single RNA transcript. This robust, high signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples. The multiplexing capabilities of both the chromogenic and fluorescent RNAscope ® assays facilitate the simultaneous visualization of multiple targets.
Here, we provide data analysis guidelines for several types of RNAscope™ staining results, including semi-quantitative and quantitative analysis methods for each of the various gene expression scenarios.
