Software solutions for all your research needs
The single-molecule sensitivity and visualization makes quantitative RNA in situ hybridization analysis a cutting edge solution. Although many labs do evaluate target probe signals manually using scoring guidelines, the tasks are time consuming and prone to subjectivity and poor reproducibility. Advanced Cell Diagnostics have partnered with Definiens, Indica Lab and Leica Biosystems to facilitate and improve quantitative scoring. These advanced image analysis solution brings objective and accurate quantification to RNA in situ hybridization. For the first time, gene expression can be measured quantitatively and interpreted by research pathologists within histopathological context.
You can select from RNAscope® SpotStudio™ Software from Definiens, Halo Software from Indica Lab or Aperio RNA ISH Algorithm by Leica Biosystems for automated image analysis solutions for RNAscope® Assays.
RNAscope® In Situ Hybridization Assay Workflow
Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.
Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.
RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.
Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.
Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.