Software solutions for all your research needs
The single-molecule sensitivity and visualization makes quantitative RNA in situ hybridization analysis a cutting edge solution. Although many labs do evaluate target probe signals manually using scoring guidelines, the tasks are time consuming and prone to subjectivity and poor reproducibility. Advanced Cell Diagnostics have partnered with Definiens, Indica Lab and Leica Biosystems to facilitate and improve quantitative scoring. These advanced image analysis solution brings objective and accurate quantification to RNA in situ hybridization. For the first time, gene expression can be measured quantitatively and interpreted by research pathologists within histopathological context.
You can select from Halo Software from Indica Lab or Aperio RNA ISH Algorithm by Leica Biosystems for automated image analysis solutions for RNAscope™ Assays.
Which Software is right for me?
Aperio RNA ISH Algorithm | HALO Software | |
|---|---|---|
Developer | Aperio RNA ISH Algorithm by Leica Biosystems | Indica Lab |
Features | Quantitative and semi-quantitative outputs Singleplex, brightfield quantification Duplex, brightfield quantification- Red/Green Count individual cells or clusters Identify subcellular location of signal (nucleus, cytoplasm) Compatible with full Aperio Digital Pathology solution | Quantitative and reproducible RNAScope spot counting Singleplex, brightfield quantification Multiplex, brightfield quantification Multiplex, fluorescent ISH quantification Cell-by-cell expression profiles High throughput computing power for faster turnaround time Whole-slide image or field-of-view image analysis Manual and automatic selection of distinct cell populations Easy to use and learn |
Assay Compatibility | RNAscope™ 2.5 HD Reagent Kit—BROWN RNAscope™ 2.5 HD Reagent Kit—RED RNAscope™ 2.5 HD Duplex Reagent Kit RNAscope™ 2.5 LS Reagent Kit—BROWN RNAscope™ 2.5 LS Reagent Kit—RED RNAscope™ 2.5 VS Reagent Kit—BROWN RNAscope™ 2.5 VS Reagent Kit—RED | RNAscope™ 2.5 HD Reagent Kit—BROWN RNAscope™ 2.5 HD Reagent Kit—RED RNAscope™ 2.5 HD Duplex Reagent Kit RNAscope™ Multiplex Fluorescent Reagent Kit RNAscope™ 2.5 LS Reagent Kit—BROWN RNAscope™ 2.5 LS Reagent Kit—RED RNAscope™ 2.5 VS Reagent Kit—BROWN RNAscope™ 2.5 VS Reagent Kit—RED |
ACD Pharma Assay Services | Image analysis service available | Image analysis service available |
Commercial Availability | Available for purchase from Leica Biosystems | Available for purchase from Indica Lab |
License Type | One-time license | Annual single or network licenses |
Aperio RNA ISH Algorithm is: For Research Use Only. Not For Use In Diagnostic Procedures.
