DNAscope Duplex Assay

Sample 2 tumor appeared to be positive for ROS1 gene rearrangement indicated by break-apart event visualized as bright blue dots

Sample 4 tumor appeared to be positive for ALK gene rearrangement indicated by break-apart event visualized as bright blue dots

ERBB2-C1/CEP17q-C2

CDKN2A-C1/CEP9q-C2

ALK WT BREAK-APART NEGATIVE CELL LINE

ALK BREAK-APART POSITIVE CELL LINE

The DNAscope assay is now discontinued. Contact bio-techne support if you have any questions.

The DNAscope chromogenic duplex (red/blue) staining allows researchers to use a standard bright-field microscope to visualize and quantify gene copy number variations (amplifications/deletions) and gene rearrangements/fusions in tissues at with spatial and morphological context at single cell resolution.

To get started with the DNAscope Duplex Assay you need:

DNAscope™ HD Duplex Reagent Kit - 324700

DNAscope™ HD Duplex Detection Reagents
DNAscope Pretreatment Reagents Kit
50x wash buffer

DNAscope Catalog Target Probes

Gene Name	Names	Catalog Number
ERBB2 (HER2)	DS-Hs-Her2-C1	1080031-C1
EGFR	DS-Hs-EGFR-C1	1080041-C1
MET	DS-Hs-MET-C1	1080051-C1
TP53	DS-Hs-TP53-C1	1080061-C1
CDKN2A	DS-Hs-CDKN2A-C1	1080071-C1
ALK	DS-Hs-ALK-BA5-C1	1080081-C1
ALK	DS-Hs-ALK-BA3-C2	1080091-C2
ROS1	DS-Hs-ROS1-BA5-C1	1080101-C1
ROS1	DS-Hs-ROS1-BA3-C2	1080111-C2
NTRK1	DS-Hs-NTRK1-BA5-C1	1080151-C1
NTRK1	DS-Hs-NTRK1-BA3-C2	1080161-C2
NTRK2	DS-Hs-NTRK2-BA5-C1	1080171-C1
NTRK2	DS-Hs-NTRK2-BA3-C2	1080181-C2
NTRK3	DS-Hs-NTRK3-BA5-C1	1080191-C1
NTRK3	DS-Hs-NTRK3-BA3-C2	1080201-C2

DNAscope Catalog Control Probes

Gene Name	Names	Catalog Number
CEP3q	DS-Hs-CEP3q-C2	1080211-C2
CEP7q	DS-Hs-CEP7q-C2	1080221-C2
CEP9q	DS-Hs-CEP9p-C2	1080231-C2
CEP12p	DS-Hs-CEP12p-C2	1080241-C2
CEP17q	DS-Hs-CEP17q-C2	1080251-C2