CDR3 for TCR
BaseScope™ Applications
Envision the ability to visualize and validate your specific TCR targets in cells or tissues with spatial and morphological context!
Complementarity determining regions (CDR) are the hypervariable regions found in the α and β chains of the T cell receptor (TCR). Due to its highly variable, short sequence that is unique to each type of T cell, CDR3 is significantly more diverse than its counterparts CDR1 and CDR2 and has been challenging to detect in the tissue context. With the BaseScope™ assay, one can now detect CDR3 sequences in intact tissues with morphological context and single molecule resolution.
FIGURE 1: BaseScope™ assay can discern between short variable sequences of the different CDR3 regions. The single ZZ probes are specifically designed to recognise sequences within the variable region of the CDR3 transcript.
FIGURE 2: Representative images of CDR3 with the BaseScope™ assay. The CCRF-specific CDR3α and CDR3β probes only recognize the TCR in CCRF T cells while they do not recognize the TCR in Jurkat T cells. Similarly, Jurkat-specific CDR3α and CDR3β probes only recognize the TCR in Jurkat T cells and do not recognize the TCR in CCRF T cells. Yellow arrows indicate positive signal.
