RNAscope™ HiPlex Pro for COMET™

RNAscope™ HiPlex Pro was developed by ACD and Lunaphore to detect up to 12 RNA targets in a method that is optimized for RNA and protein co-detection on Lunaphore’s hyperplex platform, COMET™. Combining RNAscope HiPlex Pro with seqIF™ (sequential immunofluorescence) on COMET provides end-users with a fully-automated, same-section assay integrating multiplex RNA and multiplex protein data without compromising panel flexibility or sensitivity. The combined workflow allows users to select any 12 RNAscope targets and up to 24 IF targets using off-the-shelf, non-conjugated primary antibodies. The assay is designed to be compatible with current procedures on COMET using a unique protease-free pre-treatment to promote streamlined, spatial multiomic data acquisition with subcellular resolution.