The 2024 Art of RNAscope™ Image Contest
Get in touch with your inner artist and see more in VIVID color using the full potential of the RNAscope Assay.
Share your RNAscope, BaseScope™, miRNAscope™ HiPlex™, DNAscope™ and Multiplex or Co-Detection imagery with us and we will showcase these images in our ACD Image Gallery and submit them to our ACD 2024 Calendar Contest.
It’s simple! Complete the form below to submit and upload your images.
All images selected for inclusion in the 2024 Art of RNAscope Calendar will be printed on canvas and awarded to the submitter.
Please read and agree to the terms and conditions prior to submission.
Thank you for participating. We look forward to sharing in your creativity!
*Note: last date for submission will be December 15, 2023.
Now you can see any RNA
With RNAscope™ in situ assays you can visualize
Any RNA, in ANY species, in ANY tissue.
Any RNA, in ANY species, in ANY tissue.
Click on the image galleries below to view examples of RNA visualization with our RNAscope products. Each gallery contains multiple examples of gene specific in situ RNA detection achieved using one of your RNAscope Technology Assays.
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Neuroscience
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Detection of G protein-coupled receptors (GPCRs) in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples: Chrm3 (Red) and Drd2 (Green). Cells are counterstained with DAPI. -
Detection of the effector immediate early gene and plasticity marker Arc (Green) and GPCR Drd1 (Red) in mouse brain striatum using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples. -
Detection of G protein-coupled receptors (GPCRs) in mouse brain striatum using RNAscope™2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin. -
Detection of transcription factor immediate early gene and activity marker Cfos (Green) and GPCR Chrm3 (Red) in mouse hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen samples. Cells are counterstained with DAPI -
Detection of lncRNAs in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples: Neat1 (Red) and Malat1 (Green). Cells are counterstained with DAPI. -
Fluorescent detection of ion channels. Detection of Asic1 (Green) and Kcnj3 (Red) in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples. -
Visualization of two distinct striatal neuronal populations expressing either Drd1 (Red) or Drd2 (Green) in mouse brain striatum using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples. Cells are counterstained with DAPI. -
Detection of G protein-coupled receptors (GPCRs) in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples: Cnr1 (Green) and Drd1 (Red). Cells are counterstained with DAPI. -
Detection of different cell types in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen samples: Rbfox3 (neurons, Green), Aif1 (microglia, Red) and Gfap (astrocytes, White). Cells are counterstained with DAPI. -
Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope™2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin. -
Detection of G protein-coupled receptors (GPCRs) in mouse brain hippocampus using the RNAscope™ Multiplex Fluorescent assay on Fresh Frozen tissue samples: Cnr1 (Green) and Drd1 (Red). Cells are counterstained with DAPI. -
Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope™2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin. -
Three-plex detection of glutaminergic neurons, Vglut1 (Red), Vglut2 (Green), and GABAergic neurons Vgat (white) expression in the FFPE mouse brain with RNAscope™ Multiplex Fluorescent Assay v2. Nuclei were labeled with DAPI (Blue). -
RNA expression of Sc17a6 (Vglut2)(green), SLc17a7 (Vlgut1) (red) and Slc32a1 (white) in mouse brain fresh frozen tissue using RNAscope LS Multiplex Fluorescent Assay -
Expression of Omp(r) andOlfr73(g) in mouse brain fresh-frozen tissue, RNA in situ hybridization (ISH) using RNAscope™ Multiplex Fluorescent Assay -
Neuronal Expression of SUSD4 in Mouse Hippocampus -
293T_Positive probe -
Vagal duel -
Neurons by Night -
Neuronal Subtype Spatial Heterogeneity within the Claustrum -
Neuronal Subtype Spatial Heterogeneity within the Claustrum -
NaV1.1 in NFH+ DRG Neurons -
Sodium Channel Beta Subunit Expression in DRG Neurons -
NaV1.1 in NFH+ DRG Neurons -
Tropism of SARS-Cov-2 for Human Cortical Astrocytes -
Pericyte puncta in developing human brain -
RNAscope HiPlex and Alzheimer's disease -
Embryonic Mouse Craniofacial Development -
Co-expression of cholinergic marker gene within LC-NE neurons. -
Brain cortical development -
The amazing world of non-coding RNAs in the mouse brain
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Single-Cell
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Single Cell Analysis - Simultaneous detection of 3 intestinal cell populations -
Single Cell Analysis - Spatial Validation of Single Cell Transcriptome -
Single Cell Analysis - Detection of different cell populations simultaneously in mouse brain -
Single Cell Analysis - Detection of glutamatergic and GABAergic neurons in mouse brain (Magnified) -
Single Cell Analysis - Detection of glutamatergic and GABAergic neurons in mouse brain -
Identification of distinct intestinal cell populations - Pericyte puncta in developing human brain
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Stem Cells
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Mouse Intestine FFPE Tissue: Ppib mRNA expression using the RNAscope™ LS Reagent Kit‒RED -
Detection of Olfm4 crypt base columnar (CBCs) stem cells with RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN. -
Expression of LGR5 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN. -
Rainbow trout intestinal stem cells -
Neuron progenitors in the chicken neural tube -
The avian mandibular skeleton -
Musculoskeletal anatomy of the embryonic chicken limb -
Hedgehog pathway expression in a developing chicken hindgut -
Distribution of CBSV in resistant cassava stem tissue -
Distribution of CBSV (red dots) and SE cells (green ) in a susceptible cassava tissue sections -
Stary Stary Night -
Profiles of osteogenesis in the embryonic murine palate -
Spatial organization of IgM and CXCR5 expressing cells in threespine stickleback splenic tissue
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Oncology
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HPV expression in human head and neck cancer cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Ventana Assay - BROWN -
Expression of FGFR1 RNA (brown dots) in human ovarian cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of EGFR RNA (red dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of SPP1 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of CD19 RNA (red dots) in human lymphoma tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of IGF2 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of EGFR RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of ERBB3 RNA (red dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of IDH2 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of PGR RNA (red dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of SHH RNA (red dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of GATA3 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN Expression of PDL1 RNA (brown dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using automated -
Expression of PDL1 RNA (brown dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Leica Assay-BROWN -
Expression of TP63 RNA (red dots) in human prostate cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of GATA3 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
CD274 mRNA expression in human renal cell carcinoma tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of ERBB3 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IGF2 RNA (brown dots) in human esophageal cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Leica Assay-BROWN -
Expression of CCND1 RNA (red dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of NRG1 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IGF1 RNA (brown dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Leica Assay-BROWN -
Expression of KIT RNA (red dots) in human melanoma tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of AREG RNA (brown dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of MMP9 RNA (red dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of FGFR3 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of BAP1 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of PDL1 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Leica Assay-BROWN -
Expression of FGFR1 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
CD274 mRNA expression in human non-small lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of S100P RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of MMP9 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of BRCA1 RNA (red dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of HER2 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of NRG1 RNA (red dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of PCA3 RNA (brown dots) in human prostate cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of SHH RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IL6 RNA (red dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Four-plex detection of biomarker and checkpoint markers in FFPE lung cancer tissues stained with RNAscope™ Multiplex Fluorescent Assay v2 and RNAscope™ 4-Plex Ancillary Kit: KRT19 (green), CD8A (yellow), CD4 (red), CD68 (white). -
Expression of ERBB3 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
mRNA expression in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of ANXA10 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of HGF RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Leica Assay-BROWN -
Expression of RRM1 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of LOXL2 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of TACC3 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of FGFR1 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of LOXL2 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of FGF2 RNA (red dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of MMP9 RNA (red dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of TACC3 RNA (brown dots) in human bladder cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
mRNA expression in human cervical cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of CD274 RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of DLL4 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of ERBB3 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IL6 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IL6 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of VEGFR2 RNA (brown dots) in human renal cell cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of SPP1 RNA (brown dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of IL6 RNA (red dots) in human lung cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Expression of LGR5 RNA (brown dots) in human colorectal cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
Expression of TP63 RNA (red dots) in human breast cancer tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-RED -
Gata6 expression -
Gata6 expression -
Interleukin 8 Expressing Cells in the Human Pancreas -
Shh overexpression drives tumor progression in pancreatic cancer -
All Along the Invasive Front -
Oh Christmas Tree -
Terrible Wnt - Combined IHC with RNAscope showing stromal cells (red) expressing Wnt Ligands (yellow). -
LINC00355-Menin Colocalization in Cells in Late-Stage Relapse Breast Cancer Chest Wall Patient Sample - Stary Stary Night
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Tumor heterogeneity under the RNAscope
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Infectious Diseases
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HCV-uninfected (left) or HCV-infected (right) HuH-7 cells were hybridized with probes targeting HCV mRNA (green). Cells were costained with 18S rRNA target probes (red) as an internal control for assay success. Nuclei were counterstained with DAPI (blue). -
HPV E6/E7 expression in human head and neck cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
HPV expression in human head and neck cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope™ Ventana Assay - BROWN -
HPV E6/E7 expression in human head and neck cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
HPV E6/E7 expression in human head and neck cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope™ 2.0 HD Reagent Kit-BROWN -
HIV in human lung tissue -
Detection of HIV RNA in human CD3+ T cells in the spleen of humanized BLT mice using RNAscope 2.0 HD Reagent Kit-RED. RNAscope ISH was followed by colorimetric immunohistochemistry for human T cells using mAb to human CD3, and nuclei were counterstained -
Detection of SIV using non-isotopic ISH compared to the RNAscope ISH (red). Data courtesy of Dr. Jacob Estes of NCI -
Detection of Zika viral genomic RNA with RNAscope probe #464531 (red punctate dots). The Vero cells sample was infected with ZIKV, MR766 strain. Data courtesy of Dr. Noriko Nakajima, National Institute of Infectious Disease, Japan -
Detection of Zika viral cRNA (- strand) with RNAscope™ probe #464541 (red punctate dots). The Vero cells sample was infected with ZIKV, MR766 strain. Data courtesy of Dr. Noriko Nakajima, National Institute of Infectious Disease Japan -
Human Duodenum -
SARS-CoV-2 identification in a placental tissue of a COVID-19 positive mother. - Distribution of CBSV in resistant cassava stem tissue
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Distribution of CBSV viral RNA in whole-mount cassava leaf. - Distribution of CBSV (red dots) and SE cells (green ) in a susceptible cassava tissue sections
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Distribution of CBSV and EACMV in cross-sections of susceptible cassava leaf -
Distribution of CBSV and UCBSV in cross-sections of susceptible cassava stem -
Localization of CBSV in susceptible cassava leaf tissue sections -
RSV preferntially infects ciliated cells
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Dual ISH-IHC
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Dual ISH-IHC - Detection of OLFM4 using RNAscope technology and Lyzozyme using IHC -
Human lung cancer with human PPIB positive control mRNA probe in red, CAM5.2 acidic cytokeratin immunohistochemical staining in green with DAPI as counterstain. -
Dual ISH-IHC. Detection of alpha-smooth muscle actin (SMA) using IHC (green) and Olfr558 using RNAscope technology (red)
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2021/2 Calendar
- Neuronal Expression of SUSD4 in Mouse Hippocampus
- 293T_Positive probe
- Gata6 expression
- Gata6 expression
- Rainbow trout intestinal stem cells
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Expression patterns of the Col2a1 and Prg4 transcripts in the postnatal tibial epiphysis - Vagal duel
- Neurons by Night
- Human Duodenum
- SARS-CoV-2 identification in a placental tissue of a COVID-19 positive mother.
- Interleukin 8 Expressing Cells in the Human Pancreas
- Shh overexpression drives tumor progression in pancreatic cancer
- Neuron progenitors in the chicken neural tube
- The avian mandibular skeleton
- Musculoskeletal anatomy of the embryonic chicken limb
- Hedgehog pathway expression in a developing chicken hindgut
- Distribution of CBSV in resistant cassava stem tissue
- Distribution of CBSV viral RNA in whole-mount cassava leaf.
- Distribution of CBSV (red dots) and SE cells (green ) in a susceptible cassava tissue sections
- Distribution of CBSV and EACMV in cross-sections of susceptible cassava leaf
- Distribution of CBSV and UCBSV in cross-sections of susceptible cassava stem
- Localization of CBSV in susceptible cassava leaf tissue sections
- Neuronal Subtype Spatial Heterogeneity within the Claustrum
- Neuronal Subtype Spatial Heterogeneity within the Claustrum
- NaV1.1 in NFH+ DRG Neurons
- Sodium Channel Beta Subunit Expression in DRG Neurons
- NaV1.1 in NFH+ DRG Neurons
- All Along the Invasive Front
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Oh Christmas Tree - Oh Christmas Tree
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2022/3 Calendar
- Terrible Wnt - Combined IHC with RNAscope showing stromal cells (red) expressing Wnt Ligands (yellow).
- LINC00355-Menin Colocalization in Cells in Late-Stage Relapse Breast Cancer Chest Wall Patient Sample
- Tropism of SARS-Cov-2 for Human Cortical Astrocytes
- Stary Stary Night
- Pericyte puncta in developing human brain
- RNAscope HiPlex and Alzheimer's disease
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Gene Expression in the Developing Nodose Ganglion - Embryonic Mouse Craniofacial Development
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AGER 555 MYRF 647 DAPI 63X 1_2020-02-05T11-51-33.101 -
Human BG_488 CCL21_Cy3 CLDN5_Cy5 DAPI 63X Tile 3_Stitch_2019-12-09T18-02-41.209 -
Human BG_488 SERPINF1_Cy3 COL1A2_Cy5 DAPI 63X Tile 4_Stitch_2020-02-09T16-32-38.312 - Co-expression of cholinergic marker gene within LC-NE neurons.
- RSV preferntially infects ciliated cells
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Cytotoxic T cells in the Human Pancreas -
Axon Guidance Ligands in the Normal Pancreas - Profiles of osteogenesis in the embryonic murine palate
- Spatial organization of IgM and CXCR5 expressing cells in threespine stickleback splenic tissue
- Brain cortical development
- Tumor heterogeneity under the RNAscope
- The amazing world of non-coding RNAs in the mouse brain
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