We are studying molecular mechanisms leading to neural developmental disorders using mouse, non-human primates and human tissues. To gain further insight into up-or downstream effects for specific type molecules in neurons/glia, co-localization and cell type specific gene expression analyses are invaluable. ACD’s RNAscope in situ hybridization is a powerful tool for this purpose. In our projects, we mainly use multiplex fluorescent assays combined with confocal microscopy on sectioned mouse brains. We are extremely pleased with the high resolution and the quality of the images we obtained. We compared RNAscope to other similar products developed recently and found that RNAscope’ sensitivity is much higher, the background is much lower, and assays are much easier to perform. ACD’s service and technical team is another important asset. They are fast to answer our purchasing and technical questions via email and are always attentive to our requests. I highly recommend ACD’s RNAscope Assay for any kind of multiplex ISH assay.
Juli (Hsiao-Huei) Wu, Ph.D.