What our customers are saying:

"The identification and anatomic location of HIV-infected cells and the effects of tissue environment on virus replication and viral gene expression are all critical for evaluating the pathogenesis of HIV infection and for developing cure strategies to eliminate HIV reservoirs. RNAscope in situ hybridization (ISH) has enabled us to visualize and quantify HIV RNA with single-copy resolution. For work supported by a Federal Contract with NIAID, NIH, we are using this state-of-the-art ISH technology in combination with immunohistochemistry to characterize the tissue and cellular compartments where the virus resides in HIV-infected humanized mice."

Dr. Cheryl Stoddart and Dr. Ekaterina Maidji

http://profiles.ucsf.edu/cheryl.stoddart

http://profiles.ucsf.edu/ekaterina.maidji

UCSF School of Medicine.

Recent publication:J Neurovirol. 2015 Nov 16. PMID: 26572785.

"We are very pleased with our results using the RNAscope assay. We have been using the HD Brown Detection Kit with both ‘listed’ and custom probes, and as stated on ACD’s website we have obtained a very sensitive detection with essentially no background. Both Sales and Support staffs have been exceptional in providing assistance at all stages of the process, getting us from concept to labeled slide with timely and expert advice. I have found them to be among the best of any company that I have dealt with."

Steven H. Young, Ph.D.

Researcher

Division of Digestive Diseases

Department of Medicine, David Geffen School of Medicine

CURE: Digestive Diseases Research Center University of California, Los Angeles

"I worked with ACD running RNAscope at the bench over a year ago.  It worked so well that we bought a Leica Bond RX System in order to automate the assay and use it along with IHC for most of our projects.  ACD sent two extremely knowledgeable scientists to my lab in order to install the protocols and make certain RNAscope worked on the Bond.  They did a few runs and thoroughly trained us at the same time.  It worked beautifully and it is so nice to set it up at the end of the work day and cover slip the next morning.  The support from ACD is everything you could possibly want from a vendor.  I highly recommend giving this technology a try…gone are the days when ISH took several days to complete one run!"

Kathy Mink

Scientist II

ZymoGenetics, a Bristol-Myers Squibb Company:

"ACD’s RNAscope assay worked very well. In my first experiment I was able to visualize the expression of multiple genes in zebrafish embryos with good signal and low background. The zebrafish protocol (Gross-Thebing, Paksa and Raz 2014) was very easy to run, and I plan to scale this assay to additional genes."

Tessa Montague

Ph.D. candidate

Schier lab, Harvard University

We are studying molecular mechanisms leading to neural developmental disorders using mouse, non-human primates and human tissues. To gain further insight into up-or downstream effects for specific type molecules in neurons/glia, co-localization and cell type specific gene expression analyses are invaluable. ACD’s RNAscope in situ hybridization is a powerful tool for this purpose. In our projects, we mainly use multiplex fluorescent assays combined with confocal microscopy on sectioned mouse brains. We are extremely pleased with the high resolution and the quality of the images we obtained. We compared RNAscope to other similar products developed recently and found that RNAscope’ sensitivity is much higher, the background is much lower, and assays are much easier to perform. ACD’s service and technical team is another important asset.  They are fast to answer our purchasing and technical questions via email and are always attentive to our requests. I highly recommend ACD’s  RNAscope Assay for any kind of multiplex ISH assay.

Juli (Hsiao-Huei) Wu, Ph.D.

Assistant Professor of Research
The Saban Research Institute
Children's Hospital Los Angeles

We’ve had a great experience working with ACD. Using their service team, we are able to move faster through our testing for Phase I trial, we are very happy with the quality of data, thoroughness in the reports we receive and would  highly recommend them for ISH assay development and implementation.

Omar Kabbarah, Ph.D.

Pharma/Biopharma Industry

…I had good experience with the RNAscope® in situ hybridization assay from Advanced Cell Diagnostics. Even though I had no prior experience with RNA in situ hybridization, RNAscope® assay was fairly easy to perform and it worked for me on the first try. Of course, as I used the assay more, a few adjustments were done. I tried both the chromogenic 2.0 and multiplex fluorescent assays, and they worked well for me. I would highly recommend buying ACD’s HybEZ Oven which makes performing the assay much more convenient. The results were fairly easy to interpret because the assay background is very low. Most of the work I have done so far was using sections of the mouse eye. With the help of RNAscope® technology I was able to demonstrate expression of three different genes in three clearly defined and distinct cell layers of the eye. I plan on continuing using this assay for my future studies.…

Alexey Pronin, Ph.D.

University of Miami School of Medicine

RNAScope SpotStudio Software is very easy to use. I especially like the ability to interact with the image in the result section—to connect the quantitative result to the image of the cell in question is invaluable.

Olga Shebanova

Research Investigator
Novartis

RNAscope has streamlined in situ RNA hybridization to the point were it can become a standard technique easily implemented in most labs, even by individuals with no experience with this often difficult technique. Moreover the ability to multiplex ISH to analyze simultaneously mRNA levels of up to three different genes allowed the study of changes in gene expression in specific cell populations in the CNS in response to different experimental conditions. Also this technique has been useful to analyze mRNA sub-cellular localization, which may prove important to understand disease mechanisms in neurodegenerative diseases.

Miguel Sena-Esteves, Ph.D.

Associate Professor,
University of Massachusetts Medical School  

We used ACD’s RNAscope® in situ hybridization assay service for co-localization analysis and gene expression profiling of several RNA biomarkers. For our project, we chose to use both the RNAscope chromogenic duplex and RNAscope fluorescent duplex assays. We are very pleased with the high resolution and quality of the raw data along with their expertise and timely delivery of the report and materials. The technology is impressive and is coupled with friendly service. A positive experience overall, we would highly recommend them for ISH assay development and implementation.

Alice (Yu) Chen, Ph.D.

Principal Scientist
Biotech Industry

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