"The identification and anatomic location of HIV-infected cells and the effects of tissue environment on virus replication and viral gene expression are all critical for evaluating the pathogenesis of HIV infection and for developing cure strategies to eliminate HIV reservoirs. RNAscope in situ hybridization (ISH) has enabled us to visualize and quantify HIV RNA with single-copy resolution. For work supported by a Federal Contract with NIAID, NIH, we are using this state-of-the-art ISH technology in combination with immunohistochemistry to characterize the tissue and cellular compartments where the virus resides in HIV-infected humanized mice."
http://profiles.ucsf.edu/cheryl.stoddart
http://profiles.ucsf.edu/ekaterina.maidji
UCSF School of Medicine.
Recent publication:J Neurovirol. 2015 Nov 16. PMID: 26572785.
“I honestly can't rave about RNAscope enough. We work in ferrets and it's very unique, so we have to use all custom probes. So far all of the probes we've used, even though they're custom made and nobody has used them before, have all been hits. I tell everyone that I love RNAscope over immunoassays, or Westerns, or anything like that.”
Program in Neuroscience
University of Maryland, Baltimore
“My research is focused on looking at microglia during postnatal development in the hypothalamus to understand how they're interacting with AgRP neurons and facilitating establishment of neural circuits that control food intake. RNAscope will be a huge part in helping us understand the molecular mechanisms that may contribute to how microglia are interacting with the AgRP terminals.”
Ph.D. Candidate, Simerly Lab
Vanderbilt University | Department of Molecular Physiology & Biophysics
"Our laboratory has been working with RNAscope for several years. We have been using RNAscope for the detection of EBV traces in lymphomas and epithelial tumours, classified as negative by conventional techniques. Thanks to the sensitivity of RNAscope, we have been able to obtain very good results. Recently we were able to automate EBER and EBNA1 RNAscope probes on the Leica BOND RX system, and thanks to the ACD support team, we optimized the protocols and achieved results in a short time."
PhD Student, University of Siena
Department of Medical Biotechnologies, Pathological Anatomy
"We are very pleased with our results using the RNAscope assay. We have been using the HD Brown Detection Kit with both ‘listed’ and custom probes, and as stated on ACD’s website we have obtained a very sensitive detection with essentially no background. Both Sales and Support staffs have been exceptional in providing assistance at all stages of the process, getting us from concept to labeled slide with timely and expert advice. I have found them to be among the best of any company that I have dealt with."
Researcher
Division of Digestive Diseases
Department of Medicine, David Geffen School of Medicine
CURE: Digestive Diseases Research Center University of California, Los Angeles
"I worked with ACD running RNAscope at the bench over a year ago. It worked so well that we bought a Leica Bond RX System in order to automate the assay and use it along with IHC for most of our projects. ACD sent two extremely knowledgeable scientists to my lab in order to install the protocols and make certain RNAscope worked on the Bond. They did a few runs and thoroughly trained us at the same time. It worked beautifully and it is so nice to set it up at the end of the work day and cover slip the next morning. The support from ACD is everything you could possibly want from a vendor. I highly recommend giving this technology a try…gone are the days when ISH took several days to complete one run!"
Scientist II
ZymoGenetics, a Bristol-Myers Squibb Company:
"ACD’s RNAscope assay worked very well. In my first experiment I was able to visualize the expression of multiple genes in zebrafish embryos with good signal and low background. The zebrafish protocol (Gross-Thebing, Paksa and Raz 2014) was very easy to run, and I plan to scale this assay to additional genes."
Ph.D. candidate
Schier lab, Harvard University
We are studying molecular mechanisms leading to neural developmental disorders using mouse, non-human primates and human tissues. To gain further insight into up-or downstream effects for specific type molecules in neurons/glia, co-localization and cell type specific gene expression analyses are invaluable. ACD’s RNAscope in situ hybridization is a powerful tool for this purpose. In our projects, we mainly use multiplex fluorescent assays combined with confocal microscopy on sectioned mouse brains. We are extremely pleased with the high resolution and the quality of the images we obtained. We compared RNAscope to other similar products developed recently and found that RNAscope’ sensitivity is much higher, the background is much lower, and assays are much easier to perform. ACD’s service and technical team is another important asset. They are fast to answer our purchasing and technical questions via email and are always attentive to our requests. I highly recommend ACD’s RNAscope Assay for any kind of multiplex ISH assay.
Assistant Professor of Research
The Saban Research Institute
Children's Hospital Los Angeles
We’ve had a great experience working with ACD. Using their service team, we are able to move faster through our testing for Phase I trial, we are very happy with the quality of data, thoroughness in the reports we receive and would highly recommend them for ISH assay development and implementation.
Pharma/Biopharma Industry
…I had good experience with the RNAscope™ in situ hybridization assay from Advanced Cell Diagnostics. Even though I had no prior experience with RNA in situ hybridization, RNAscope assay was fairly easy to perform and it worked for me on the first try. Of course, as I used the assay more, a few adjustments were done. I tried both the chromogenic 2.0 and multiplex fluorescent assays, and they worked well for me. I would highly recommend buying ACD’s HybEZ Oven which makes performing the assay much more convenient. The results were fairly easy to interpret because the assay background is very low. Most of the work I have done so far was using sections of the mouse eye. With the help of RNAscope technology I was able to demonstrate expression of three different genes in three clearly defined and distinct cell layers of the eye. I plan on continuing using this assay for my future studies.…
University of Miami School of Medicine
