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  • Mar
    03
    2021 Mar 3 - Webinar: Introducing New RNA-Protein Co-Detection Assays
    Madhura Nayak, Product Manager, Advanced Cell Diagnostics, a Bio-Techne Brand
    Anushka Dikshit, Ph.D., Applications Scientist, Advanced Cell Diagnostics
    2021 Mar 3 - Webinar: Introducing New RNA-Protein Co-Detection Assays
    Madhura Nayak
    Product Manager, Advanced Cell Diagnostics, a Bio-Techne Brand
    Anushka Dikshit, Ph.D.
    Applications Scientist, Advanced Cell Diagnostics
    Outline:

    Launch Webinar: Introducing ACD's New RNA-Protein Co-Detection Assays

    Learn more about ACD’s new Co-detection Assays which will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

    Learning Objectives:
    Combining RNA and Protein enables researchers to:
     
    • Characterize cell type-specific expression
    • Identify origin of secreted proteins
    • Visualize cell surface markers with RNA of interest
    • Visualize RNA binding proteins and their target RNA
    • Dissect regulation of gene expression
    • Antibody validation and specificity confirmation
    • Expand research in oncology, neuroscience, developmental biology, infectious diseases and more
    Dates and Registration:
    March
    03
    8am PST
    11am EST
    6pm CEST
    Register Now
  • Mar
    17
    2021 Mar 17 - Oncofetal ecosystem: space, time and memory
    Ankur Sharma, Ph.D.,, Laboratory Head at Harry Perkins Institute of Medical Research and women’s cancer senior fellow/Senior lecturer at Curtin Health Innovation Research Institute (CHIRI), Curtin University, Perth, Australia.
    Anushka Dikshit, Ph.D., Applications Scientist, Advanced Cell Diagnostics
    2021 Mar 17 - Oncofetal ecosystem: space, time and memory
    Ankur Sharma, Ph.D.,
    Laboratory Head at Harry Perkins Institute of Medical Research and women’s cancer senior fellow/Senior lecturer at Curtin Health Innovation Research Institute (CHIRI), Curtin University, Perth, Australia.
    Anushka Dikshit, Ph.D.
    Applications Scientist, Advanced Cell Diagnostics
    Outline:

    This webcast will describe employing single cell genomics and spatial transcriptomics to discover a previously unexplored oncofetal reprogramming of the tumor ecosystem.

    Analysis of ~212,000 cells representing human fetal, hepatocellular carcinoma (HCC), and mouse liver revealed remarkable fetal-like reprogramming of the tumor microenvironment. Specifically, the HCC ecosystem displayed features reminiscent of fetal development, including re-emergence of fetal-associated endothelial cells and fetal-like tumor-associated macrophages.

    The speaker will describe a shared immunosuppressive oncofetal ecosystem in fetal liver and HCC and its potential implications for targeting therapeutic interventions in HCC and identifying similar paradigms in other cancers and diseases.

    Learning Objectives:
    • Identification of fetal-associated endothelial cells and macrophages in HCC
    • Shared oncofetal ecosystem between human fetal liver and HCC
    • The phenomenon of oncofetal reprogramming in the tumor ecosystem
    Dates and Registration:
    March
    17
    10am SGT
    7pm PST
    10pm EST
    3am CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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