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  • Sep
    22
    2020 Sept 22 - Knowledge Resource Series - Stem Cell Applications Webinar
    Jyoti Phatak-Sheldon, Associate Scientist - Applications, Advanced Cell Diagnostics
    2020 Sept 22 - Knowledge Resource Series - Stem Cell Applications Webinar
    Jyoti Phatak-Sheldon
    Associate Scientist - Applications, Advanced Cell Diagnostics
    Outline:

    Spatial mapping of gene expression and genetic variants in the tissue context with the RNAscope technology for Stem Cell Research

     

    Nucleic acid-based molecular detection methods have revolutionized detection of stem cell markers, offering several essential advantages such as sensitivity, specificity, and multiplexing capabilities.

    Beyond this, RNAscope ISH uniquely offers molecular detection at a single cell resolution coupled with morphological context to enable visualization of these stem cell markers in various tissue types such as organoids, frozen and FFPE tissues.

    In this webinar, we will demonstrate how RNAscope can be used for spatially mapping novel cell types and morphological markers in stem cell research

    Learning Objectives:
    • Learn about the RNAscope, BaseScope and miRNAscope assays that enable detection of a diverse range of targets.
    • Broad applications of the RNAscope technology in research areas such as Oncology, Neuroscience, Infectious disease, Developmental biology and more .
    • Compatible workflow with IHC/IF allowing detection of RNA and protein simultaneously.
    • Multiplexing capabilities enable detection of multiple targets on the same tissues section.
    Dates and Registration:
    September
    22
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Sep
    29
    2020 Sept 29: Introducing ACD's New RNA-Protein Co-Detection Assays
    Madhura Nayak, Product Manager, Advanced Cell Diagnostics
    Anushka Dikshit, Ph.D., Applications Scientist, Advanced Cell Diagnostics
    2020 Sept 29: Introducing ACD's New RNA-Protein Co-Detection Assays
    Madhura Nayak
    Product Manager, Advanced Cell Diagnostics
    Anushka Dikshit, Ph.D.
    Applications Scientist, Advanced Cell Diagnostics
    Outline:

    Learn more about ACD’s new Co-detection  Assays which will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

    Learning Objectives:

    Combining RNA and Protein enables researchers to

    • Characterize cell type-specific expression
    • Identify origin of secreted proteins
    • Visualize cell surface markers with RNA of interest
    • Visualize RNA binding proteins and their target RNA
    • Dissect regulation of gene expression
    • Antibody validation and specificity confirmation 
    • Expand research in oncology, neuroscience, developmental biology, infectious diseases and more
    Dates and Registration:
    September
    29
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Oct
    06
    2020 Oct 6 - Longitudinal Immune Profiling Reveals Key Immune Signatures Associated with COVID-19
    Madhvi Menon, PhD, Presidential Research Fellow, School of Biological Sciences, University of Manchester
    Jyoti Phatak-Sheldon , Associate Scientist, Applications Advanced Cell Diagnostics, a Bio-Techne Brand
    2020 Oct 6 - Longitudinal Immune Profiling Reveals Key Immune Signatures Associated with COVID-19
    Madhvi Menon, PhD
    Presidential Research Fellow, School of Biological Sciences, University of Manchester
    Jyoti Phatak-Sheldon
    Associate Scientist, Applications Advanced Cell Diagnostics, a Bio-Techne Brand
    Outline:

    COVID-19 pathogenesis is associated with an exaggerated immune response. However, the specific cellular mediators and inflammatory components driving diverse clinical disease outcomes remain poorly understood. In this webinar sponsored by Advanced Cell Diagnostics (ACD), Madhvi Menon will describe the longitudinal immune profiling of hospitalized patients during the peak of the COVID-19 pandemic in the UK, and the key immune signatures that associated with disease severity. Additionally, Jyoti Phatak-Sheldon will describe what researchers can learn about COVID-19 using ACD’s RNAscope in situ hybridization technology with RNA probes specific for SARS-CoV-2.

     

    Learning Objectives:
    • Immune profiling of COVID-19 patients in the UK
    • Immune signatures associated with severe COVID-19
    • Changes in immune signatures after recovery
    • RNAscope in situ hybridization technology specific for SARS-CoV-2
    Dates and Registration:
    October
    06
    11:30am PST
    2:30 pm EST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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