There is no upcoming webinars. Please check us later.

    You can watch a video or download presentation of the past webinars.

    Recorded Webinars

    New Product Launch Webinars

    Professional Assay Services Webinar Series

    Technical Support Webinar Series

    Webinars by Research Area

    Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

    How to use EZ-Batch Tray


    De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

    Endogenous Peroxidase Blocking

    RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

    Target Retrieval (Boiling)

    Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

    Protease Plus (Protease Digestion)

    Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

    Target Probe Hybridization

    ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

    Amplification (Amp1-Amp6) with Wash Step

    RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

    DAB Colormetric Reaction

    Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

    Gill's Hematoxylin Counter Stain

    Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

    Tissue Dehydration

    The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.


    The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

    Download any of the following webinar presentation documents.

    PresentationsDownload File
    Ready Set Go Getting Started with RNAscope_May 12 2015

    Ready Set Go Getting Started with RNAscope_Apr 14 2015

    RNAscope Troubleshooting Tips_June 16 2015

    RNAscope Troubleshooting Tips_July 14 2015

    Contact ACD Technical Support

    How can we help you?

    Please contact us directly, by phone or email, for any technical assistance with the RNAscope™ assay or relevant products.

    Contact Us

    Complete one of the three forms below and we will get back to you.

    For Quote Requests, please provide more details in the Contact Sales form below

    Advanced Cell Diagnostics

    Our new headquarters office starting May 2016:

    7707 Gateway Blvd.  
    Newark, CA 94560
    Toll Free: 1 (877) 576-3636
    Phone: (510) 576-8800
    Fax: (510) 576-8798



    19 Barton Lane  
    Abingdon Science Park
    OX14 3NB
    United Kingdom
    Phone 2: +44 1235 529449
    Fax: +44 1235 533420


    Advanced Cell Diagnostics China

    20F, Tower 3,
    Raffles City Changning Office,
    1193 Changning Road, Shanghai 200051


    For general information:
    For place an order:
    For product support:
    For career opportunities: