Insights into viral pathogenesis.

Determination of causation by highly sensitive and specific viral RNA detection and localization with RNAscope™ ISH.

“A method as sensitive as RNAscope assay with spatial resolution is absolutely irreplaceable for our confidence in determining viral contribution to a disease.”

Characterizing LGR5 stem cells in colorectal adenomas and carcinomas.

Overcoming the limitations of antibody-based detection methods.

“Using the RNAscope assay probe against LGR5 mRNA has allowed us to study the localization of LGR5 expression without relying on an antibody””

Realizing the potential of long noncoding RNA (lncRNA) as a cancer biomarker.

From NGS discovery to validation with RNA in situ Hybridization.

“We found RNAscope technology fast and easy to use, this technology also allows us to directly visualize gene expression in the target tissue of interest ....Also, only one 4 micron FFPE section is sufficient to give us all this information.”

Development of Prostate Cancer Personalized Medicine.

Advancing analysis of prostate tumor molecular heterogeneity by combined immunohistochemistry and novel RNA in situ hybridization.

“Given the unique probe design and detection chemistry of the RNAscope method, we were able to get results with high specificity and sensitivity for many probes that we have used in my laboratory. To date we don’t have any comparable technology for the reliable detection of RNA in FFPE tissues other than RNAscope.”

Exploring GPCR expression in the eye.

From discovery with NGS to spatial and quantitative expression confirmation with RNAscope™ technology.

“Because RNAscope can detect expression of multiple genes in parallel, it can tell us whether those genes discovered through RT-PCR are co-expressed in the same cell or not – information that is very valuable and hard to obtain by other means.”

Detecting a Novel Circovirus in Foxes with Meningoencephalitis - How custom RNAscope™ assay probes are enhancing viral understanding

The burden of infectious diseases on human health worldwide has markedly increased over the past few decades, most of these emerging events are caused by viruses originating from the animal world. Understanding the roots of viral behavior in animal hosts is therefore vital and this is a primary focus of Sarah Getu’s and Saskia Smits’s work at the Department of Viroscience, Erasmus MC. In this interview, they explain how the creation of a custom RNAscope™ in situ hybridization (ISH) probe allowed them to localize a newly discovered circovirus to neurologic lesions in foxes, providing valuable information to support causation.

Shedding light on human cerebral cortical folding and channelopathies.

“The RNAscope assay was very helpful in discovering this, not only because we were able to confidently discern between the different sodium channels, but also because we could use the RNAscope Multiplex Fluorescent kit v2 in situ assay to co-localize gene expression with cell types.”

Harnessing spatial biology to uncover the cellular landscape of colorectal cancer

"Being able to spatially resolve cells in tissue using tools like RNAscope™ …will have a lot of clinical relevance in the future… that’s where the real power of this can be seen going from bench to bedside."

Unlocking the power of organoids in personalized medicine.

Organoids are used to study many diseases that otherwise could not have been studied using traditional cell lines.  Using RNAscope™ ISH technology to understand spatial gene expression and heterogeneity at the single-cell level in sub populations of enteroendocrine cells in the gut epithelium. It is 'key technologies like RNAscope used after scRNAseq that is enabling us to visualize the genes in the context of the whole tissue...and pave the way for therapeutic approaches to intestinal diseases".