Tissue detachment
Various samples undergo detachment during reparation for RNAscope™ assay. Based on the sample type certain steps can be implemented. The following figures illustrate the steps to be altered.
FFPE Samples
Before
Suboptimal tissue preparation
Baked for 1 hour at 60°C
After
- Prepare tissue samples according to recommended procedures
- Bake slides for a longer time (up to overnight)
- Reduce boiling time
Tips for troubleshooting tissue detachment in FFPE samples:
- Always use Superfrost Plus Slides® (Fisher Scientific, Cat #12-550-15)
- Bake/dry slides in active air circulating oven (NOT HybEZ™ oven)
- Maintain mild boiling of target retrieval at ~100°C
