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Tissue detachment

Various samples undergo detachment during reparation for RNAscope® assay. Based on the sample type certain steps can be implemented. The following figures illustrate the steps to be altered.

FFPE Samples 


FIGURE 7. FFPE samples. Additional baking steps are added to the sample preparation workflow. For sample detachment after baking/deparaffinizaton step or after target retrieval step, a baking step is added for 30 min at 60°C (I) or (II), respectively.

Before

Suboptimal tissue preparation

Baked for 1 hour at 60°C

After

  • Prepare tissue samples according to recommended procedures​
  • Bake slides for a longer time (up to overnight)
  • Reduce boiling time
Tips for troubleshooting tissue detachment in FFPE samples:
  • Always use Superfrost Plus Slides® (Fisher Scientific, Cat #12-550-15)
  • Bake/dry slides in active air circulating oven (NOT HybEZ™ oven)
  • Maintain mild boiling of target retrieval at ~100°C
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