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Tissue detachment

Various samples undergo detachment during reparation for RNAscope® assay. Based on the sample type certain steps can be implemented. The following figures illustrate the steps to be altered.

FFPE Samples 

FIGURE 7. FFPE samples. Additional baking steps are added to the sample preparation workflow. For sample detachment after baking/deparaffinizaton step or after target retrieval step, a baking step is added for 30 min at 60°C (I) or (II), respectively.


Suboptimal tissue preparation

Baked for 1 hour at 60°C


  • Prepare tissue samples according to recommended procedures​
  • Bake slides for a longer time (up to overnight)
  • Reduce boiling time
Tips for troubleshooting tissue detachment in FFPE samples:
  • Always use Superfrost Plus Slides® (Fisher Scientific, Cat #12-550-15)
  • Bake/dry slides in active air circulating oven (NOT HybEZ™ oven)
  • Maintain mild boiling of target retrieval at ~100°C
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Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798



19 Barton Lane  
Abingdon Science Park
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420



Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

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