Comparison of mild and standard pretreatment conditions for recommended positive control probe per tissue type
The following pages show positive control probe images with standard and mild pretreatment conditions. Optimal pretreatment condition is indicated for each tissue type.
2.2.1 Comparison of mild and standard pretreatment conditions for recommended positive control probe per tissue type
Positive control probe: Hs-PPIB
Tissue: Spleen
Pretreatment: Standard
SCORE 1-2+*Pretreatment: Mild
SCORE 1-2+*Observation: Loss of cell boundary using standard pretreatment.
Positive control probe: Hs-PPIB
Tissue: Lymph node
Pretreatment: Standard
SCORE 1-2+, 4+*Pretreatment: Mild
SCORE 1-2+, 4+*Observation: Loss of cell boundary using standard pretreatment. Signal: Mild ≥ Standard.
Positive control probe: Hs-PPIB
Tissue: Tonsil
Pretreatment: Standard
SCORE 2+, 3Pretreatment: Mild
SCORE 2+, 4+*Observation: Loss of cell boundary using standard pretreatment. Signal: Mild > Standard
Positive control probe: Hs-POLR2A**
Tissue: Retina
Pretreatment: Standard
SCORE 1-2+Pretreatment: Mild
SCORE 2+Observation: Loss of cell boundary using standard pretreatment. Signal: Mild ≥ Standard.
*A few cells have higher PPIB signals **Retina expresses low level of PPIB
