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The following pages show positive control probe images with standard and mild pretreatment conditions. Optimal pretreatment condition is indicated for each tissue type.

2.2.1 Comparison of mild and standard pretreatment conditions for recommended positive control probe per tissue type

Positive control probe: Cl-POLR2A

Tissue: Spleen

Pretreatment: Standard
SCORE 1+
Pretreatment: Mild
SCORE 1+

Observation: Loss of cell boundary using standard pretreatment.

Positive control probe: Cl-POLR2A

Tissue: Lymph node

Pretreatment: Standard
SCORE 1-2+
Pretreatment: Mild
SCORE 1-2+

Observation: Loss of cell boundary using standard pretreatment.

Positive control probe: Cl-POLR2A

Tissue: Thymus

Pretreatment: Standard
SCORE 1-2+
Pretreatment: Mild
SCORE 1-2+

Observation: Loss of cell boundary using standard pretreatment.

Positive control probe: Cl-POLR2A

Tissue: Retina (eye)

Pretreatment: Standard
SCORE 2+
Pretreatment: Mild
SCORE 1-2+

Observation: Standard pretreatment causes more cell detachment and reduced signals.

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