The RNAscope™ assay can be performed manually or on fully automated staining systems, including the Discovery Ultra and Discovery XT Automated IHC/ISH slide staining systems from Roche Tissue Diagnostics and Leica Biosystems’ BOND RX Research Advanced Staining System (Table 1). The RNAscope procedure can be completed within a single day and consists of the following steps outlined below.
ACD also offers Professional Assay Services to make this technology available for customers preferring to outsource tissue-based projects. The assay provides visualization of each individual RNA molecule as a punctate dot under a standard bright-field microscope or fluorescent microscope. These dots can be quantified by counting the number of signal dots in individual cells, either manually or by using image analysis tools, such as HALO™ software (Indica Labs), and Aperio RNA ISH Algorithm (Leica Biosystems).
