Other issues
Successful implementation of the RNAscope™ assay is directly linked to hybridization environment. The ACD HybEZ™ Hybridization System and its ability to accurately keep the temperature stable is essential to success of the RNAscope™ assay.
The HybEZ™ Oven is a simple, easy-to-use, low-profile benchtop hybridization oven that provides superior conditions for RNA-ISH, and is the only hybridization oven for which ACD can provide our customers with our RNAscope™ assay performance guarantee. The HybEZ™ Oven provides a gasket-sealed, temperature-controlled humidifying chamber necessary for optimized RNAscope™ assay performance.
No staining observed
Problem
Hybridization conditions not optimal
Solution
Use HybEZ™ Hybridization System
Corrected Staining
No staining or less than 1 dot to every 10 cells
