Positive and negative control probe data using optimal conditions per tissue type
The following pages show the three positive controls (Ppib, Polr2a, Ubc) and the negative control images using optimal pretreatment condition. Recommended positive control probe is indicated for each tissue type.
2.1.2 Positive and negative control probe data using optimal conditions per tissue type
Tissue: Spleen
Pretreatment: Mild
Control probe: Rn-Ppib
SCORE 2+, 4+*Control probe: Rn-Polr2a
SCORE 1-2+Control probe: Rn-Ubc
SCORE 4+Control probe: DapB
SCORE 0
*A few cells have higher Ppib signal
**Rn-Polr2a staining is more evenly distributed
Tissue: Lymph node
Pretreatment: Mild
Control probe: Rn-Ppib
SCORE 2+, 3+*Control probe: Rn-Polr2a
SCORE 1-2+*Control probe: Rn-Ubc
SCORE 3+Control probe: DapB
SCORE 0
*Rn-Polr2a staining is more evenly distributed
