Choosing appropriate control probes
Negative controls
ACD’s universal negative control probe ensures that there is no background staining related to the assay and that the tissue specimen is appropriately prepared. The probe targets the dapB gene (GenBank accession #EF191515) from Bacillus subtilis strain SMY, a soil bacterium, and is suitable for all samples. Alternatively, probes from an unrelated species, for instance, a zebrafish probe on human tissue, can be used.
Positive controls
Positive control probes should be selected depending on the level of expression of the target gene.
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