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Positive and negative control probe data using optimal conditions per tissue type

The following pages show the three positive controls (PPIB, POLR2A, UBC) and the negative control images using optimal pretreatment condition. Recommended positive control probe is indicated for each tissue type.

2.3.2 Positive and negative control probe data using optimal conditions per tissue type

Tissue: Lymph node

Pretreatment: Mild

Control probe: Hs-PPIB
SCORE 2+, 4+*
Control probe: Hs-POLR2A
SCORE 1+
Control probe: Hs-UBC
SCORE 3+
Control probe: DapB
SCORE 0
 

*Hs-POLR2A staining is more evenly distributed

Tissue: Tonsil

Pretreatment: Mild

Control probe: Hs-PPIB
SCORE 2+, 4+*
Control probe: Hs-POLR2A
SCORE 1+
Control probe: Hs-UBC
SCORE 3+
Control probe: DapB
SCORE 0
 

*Hs-POLR2A staining is more evenly distributed

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