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High background

Unexpected staining patterns can commonly be due to suboptimal digestion conditions. Use examples below to determine possible issue and solution.

Nuclear Background


Standard pretreatment (15 min target retrieval + 30 min protease) on Mm kidney

Problem

Pretreatment conditions not optimal

  • Tissue is over-digested
  • Tissue is under-digested
7 min target retrieval + 30 min protease on Mm kidney

Solution

Optimize pretreatment conditions:

  • Decrease boiling and/or protease if tissue is over-digested
  • Increase boiling and/or protease if tissue is under-digested

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