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High background

Unexpected staining patterns can commonly be due to suboptimal digestion conditions. Use examples below to determine possible issue and solution.

Nuclear Background

Standard pretreatment (15 min target retrieval + 30 min protease) on Mm kidney


Pretreatment conditions not optimal

  • Tissue is over-digested
  • Tissue is under-digested
7 min target retrieval + 30 min protease on Mm kidney


Optimize pretreatment conditions:

  • Decrease boiling and/or protease if tissue is over-digested
  • Increase boiling and/or protease if tissue is under-digested

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Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798



19 Barton Lane  
Abingdon Science Park
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420



Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

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