Scenario description
In this scenario the target of interest is specifically expressed in a (sub)population of cells or a particular region (Figures 8 and 9). In this scenario both the expression level and the percent of cells expressing the target at different levels could be of importance.
Figure 7. Region-specific expression pattern for Lgr5 in mouse colon using the RNAscope ® 2.5 HD BROWN assay.
Figure 8. Subpopulation-specific expression for Vglut1 (red) and Vglut2 (green) on glutamatergic neuronal populations and Vgat (white) on GABAergic neurons in normal mouse brain using the RNAscope ® LS Multiplex Fluorescent Assay. Cells are counterstained with DAPI (blue).
Analysis guidelines
In the scenario that a biomarker or target is expressed in a specific (sub)population of cells or a specific region, one can specifically analyze this relevant cell (sub)population or region according to methodologies #1 and #2. H score (methodology #3) can also be derived, but it is recommended to use software analysis. Furthermore, percentage positive cells can be assessed based on number of cells with >1 dot/cell.
