Target expression in a rare cell type
Scenario description
In this scenario a small number of cells show expression for a particular target and therefore identifying the number of cells that express the target of interest could be more relevant than the average expression level per cell (Figure 19).
Figure 19. CD8-positive T cells (red) infiltrating a PD-L1-positive (green) tumor region in lung cancer using the RNAscope ® 2.5 HD Duplex assay.
Analysis guidelines
To determine the rare cell expression, one can qualitatively and quantitatively assess the expression pattern by using methodologies #1 and #2.
