Cell population interactions
Scenario description
In this scenario specific cell type populations and their spatial relationship to one another are being investigated (Figure 20). Hereto, one can assess the number of cells expressing the targets of interest and their proximity to one another within the tissue environment.
Figure 20. The spatial relationship of CD45-positive cells (red) with PD-L1-positive (green) tumor cells in lung cancer using the RNAscope ® 2.5 HD Duplex assay.
Analysis guidelines
One can qualitatively and quantitatively assess the expression pattern by using methodologies #1 and #2.
