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RNAscope® technology

The RNAscope ® assay provides a powerful method to detect gene expression within the spatial and morphological context of the tissue. The proprietary “double Z” probe design in combination with advanced signal amplification techniques enables highly specific and sensitive detection of target RNA, which can be visualized as a dot, with each dot representing a single RNA transcript. This robust, high signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples. The multiplexing capabilities of both the chromogenic and fluorescent RNAscope ® assays facilitate the simultaneous visualization of multiple targets.

Here, we provide data analysis guidelines for several types of RNAscope® staining results, including semi-quantitative and quantitative analysis methods for each of the various gene expression scenarios.

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Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798



19 Barton Lane  
Abingdon Science Park
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420



Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

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