Scenario description
In this scenario the target of interest is expressed in two or more distinct cell types (Figure 6). In this scenario both the expression level and the percent of cells expressing the target at different levels could be of importance.
Figure 6. Detection of Ctnnb1 (β-catenin) expression in mouse colon with TNBS-induced colitis. Here we can observe Ctnnb1 expression in smooth muscle cells (muscularis propria) (A), colonic epithelial cells (B), and inflammatory cells (lymphoid aggregate) (C).
Analysis guidelines
In the scenario that a biomarker or target is expressed in two or more different cell types, one can analyze both cell types independently according to methodologies #1 and #2. H score (methodology #3) can also be derived, but it is recommended to use software analysis. The analyses can also be combined to calculate the percentage positive for each cell type of interest (Table 2).
