Scenario description
In the homogeneous target expression scenario cells display relatively uniform staining for the target RNA, indicating homogeneous gene expression among the same cell type (Figure 1). The overall homogeneous target expression level of the entire cell population throughout a tissue section can be assessed by measuring the average number of dots per cell.
Figure 1. Homogeneous expression of MICA (A) and MICB (B) in human ovarian cancer tissue using the RNAscope ® 2.5 HD BROWN assay.
Analysis guidelines
The average number of dots per cell can be measured by using either a semi-quantitative histological scoring methodology (called methodology #1) based on the ACD scoring criteria shown in Table 1 or image-based quantitative software analysis tools (called methodology #2) illustrated in Figure 2. According to methodology #1 guidelines, MICA and MICB expression are scored as 1 and 2, respectively.
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