Species-specific positive probes and negative control probes are needed to run with human or mouse control slides for a quality control check of the technique and with your sample for the sample or RNA quality control check.
ACD provides positive control probes that target species-specific housekeeping genes with different expression levels. Negative control probes target the bacteria DapB gene.
Careful selection of the right positive control is strongly recommended. The expected expression level of your target RNA is the key criteria to consider as your positive control probe should have similar expression profile. See the overview page for details.
The negative and positive control probes in channel 1 can be used for both RNAscope™ 2.5 HD BROWN or RNAscope™ 2.5 HD RED assay, as both assays function in Probe Channel 1. The names for these probes do not contain a C1 or Channel 1 in its names.
