Species-specific positive probes and negative control probes are needed to run with human or mouse control slides for a quality control check of the technique and with the sample for a Sample or RNA quality control check. ACD provides positive control probes that target specie-specific housekeeping genes and negative control probes that target the bacterial DapB gene.
Careful selection of the right positive control is strongly recommended. The expected expression level of your target RNA is the key criteria to consider as your positive control probe should have a similar expression profile. See the overview page for more details.
The same control probes can be used for both RNAscope 2.5 VS Reagent Kit—BROWN and RNAscope 2.5 VS Reagent Kit—RED, as both function in Probe Channel 1. The names for these probes will contain "2.5 VS Control Probes".
