Whole mount in situ hybridization (ISH) is a very informative approach for visualizing gene expression patterns over the entire embryo or tissue 3D structure. However, conventional whole mount ISH procedures are lengthy and require extensive optimization. During the last few years, our customers have shown very innovative approaches to applying the RNAscope™ ISH technology on whole mount samples:

RNAscope^TM Technology for Cardiovascular and Skeletal Muscle Research

RNAscope^TM Technology in Liver Research

Neural Development

Post-mortem Brain Tissue

Studies on the postmortem human brain have become a very important tool for understanding the neurobiology of neurodegenerative diseases or psychiatric disorders. Histology and immunohistochemistry are sometimes challenging due to technical limitations

mRNA in post mortem tissues is rapidly degraded after death and
formalin-based fixatives and embedding techniques can impair immunoreactivity. 

Detection, characterization and (co-) localization of neuronal cell types and subcellular target localization

Detection of G protein-coupled receptors (GPCRs) - 
Detection of mRNA in the nervous system when no (reliable) antibodies are available 

Unique detection and visualization of EGFRVIII splice variant in FFPE sample

With our wide portfolio of ready-to-use probes, ACD provides a broad range of high-quality RNAscope probes for your RNA ISH needs in Anatomical Pathology. We have categorized the key RNAscope probes into thirteen Anatomical Pathology panels, based upon most common tumor, tissue and infectious disease types. Each panel includes RNAscope probes designed to enhance assessment and characterization of target markers in the organ-specific disease states.