RNAscope™ 2.5 LS Reagent Kit – BROWN

RNAscope™ 2.5 LS Brown - Breast Cancer - FGFR3

RNAscope™ 2.5 LS Brown - Breast Cancer - FGFR3

RNAscope™ 2.5 LS Brown - Breast Cancer - NRG1

RNAscope™ 2.5 LS Brown - Breast Cancer - NRG1

RNAscope™ 2.5 LS Brown - Colon Cancer - PILR2A

RNAscope™ 2.5 LS Brown - Colon Cancer - PILR2A

RNAscope™ 2.5 - Liver Cancer - PPIB

RNAscope™ 2.5 - Liver Cancer - PPIB

The RNAscope 2.5 LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The RNAscope 2.5 LS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH.

RNAscope 2.5 LS Reagent Kit—BROWN is a very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope 2.5 LS Reagent Kit—BROWN is designed to provide more intense Diaminobenzidine (DAB) staining—ideal when low copy target gene expression is anticipated (1–20 copies per cell). The stained slides are visualized with brightfield microscopes. 

These new RNAscope 2.5 LS reagents require the new RNAscope 2.5 LS Probes for optimal performance. Probe designs are identical to previous version, however the RNAscope 2.5 LS probes are manufactured for optimal performance with RNAscope 2.5 LS reagent chemistry.

The RNAscope LS Automated Assay requires the following from Leica Biosystems (LeicaBiosystems.com):

CAT #Product Name and DescriptionQuantity
OPT9049BOND Titration Kit10 containers, 50 inserts
OP79193BOND Open Containers (7 mL)10 pack
OP309700BOND Open Containers (30 mL)10 pack
S21.4611BOND Universal Covertiles™160 pack
AR9961 & AR9640BOND Epitope Retrieval Solution 1 & 21L (RTU) each
S21.1971BOND Mixing Stations6 vials
AR9222BOND Dewax Solution1L (RTU)
AR9590BOND Wash Solution (10x concentrate)1L
DS9800BOND Polymer Refine Detection1 kit
CS9100BOND Aspirating Probe Cleaning System1 system, 15 cleans


RNAscope 2.5 LS Automated Assay-BROWN Workflow Requires the following from ACD:

  • RNAscope 2.5 LS Target Probes (Catalog or Made-to-Order LS Probes)
  • RNAscope 2.5 LS Control Probes (Select species-specific LS positive control probes or packs of probes and LS negative control probes)
  • RNAscope Control Slides (optional)
  • RNAscope 2.5 Leica Assay Reagent Kit—BROWN 

Product Retirement:

Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).

Please refer to the FAQ for more information about 2.0 discontinuance.

More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope New Product Development- learn more about RNAscope 2.5 product line” here

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