The RNAscope 2.5 LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The RNAscope 2.5 LS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH.
The RNAscope 2.5 LS Reagent Kit-RED with it Fast Red dye offers a higher contrast and is the first choice in in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed, ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.
These new RNAscope 2.5 LS reagents require the new RNAscope 2.5 LS Probes for optimal performance. Probe designs are identical to previous version, however the RNAscope 2.5 LS probes are manufactured for optimal performance with RNAscope 2.5 reagent chemistry.
