The rapid and expansive field of stem cell biology has demonstrated the remarkable capabilities of these cells to self-renew, differentiate and reprogram. Yet the field continues to expand with studies trying to elucidate stem cell populations, characterize stem cell markers and identify the signals secreted from stem cells. RNAscope™ in situ hybridization (ISH) technology enables cell-specific localization of RNA transcripts quickly and precisely for markers of stem cell populations.
The RNAscope™ assay can be used to

A tumor is a complex organ consisting of many cell types. Localization of an alteration in gene expression to specific cell types provides the first link to cellular function. The pursuit of personalized medicine through technologies like microarrays and next-generation sequencing has generated a wealth of novel biomarkers, which promise to improve cancer diagnosis and patient stratification. However, for many of these biomarkers, we do not know which specific cell types in the tumor express them. This limits our ability to fully understand their biological relevance.