NEW! Introducing our protease-free pretreatment workflow for sequential RNA-Protein detection

Analysis of both RNA and protein provides invaluable information for understanding the regulation of expression. Both ISH and IHC technologies share common features that make them unique amongst the protein and RNA detection technologies.

In the most recent statistics from the GENCODE project (v29, October 2018), the human genome contains 23,643 non-coding RNA (ncRNA) genes, surpassing the number of protein-coding genes (19,940). Of the non-coding RNA species, some 30% (7,577) are less than 200 bases long, termed as small non-coding RNA. They comprise of transfer RNA (tRNA) and ribosomal RNA (rRNA), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs and piRNA.

Evidence for transcriptionally active HPV oncogenes E6/E7 is regarded as the gold standard for presence of clinically relevant high-risk human papillomavirus (HPV), but detection of HPV E6 E7 mRNA can be challenging using conventional techniques. As a causal agent in head and neck squamous cell carcinoma (HNSCC), it is critical that the detection method enable pathologist review of tissue morphology and be of the highest specificity and sensitivity for accurate assessment of within the tissue microenvironment of FFPE specimens.