Control slides are essential to verify the assay conditions. ACD strongly recommends that all new users begin their RNAscope™ or BaseScope™ Assay experiments by selecting human or mouse control slides and running the assay on these slides with positive and negative control probes. This first run would serve as an assay control check before performing runs using your target probes and samples. Please note that control slides for BaseScope™ and RNAscope™ are identical and named RNAscope™ Control Slides.
RNAscope™ Control Slides for RNAscope™ and BaseScope™ Assays
Two varieties of control slides are offered: human control slides containing FFPE cultured cell pellets of human HeLa cells and mouse control slides containing FFPE cultured cell pellets from mouse NIH 3T3 cells.
In addition to purchasing the control slides, you will need to purchase species-specific positive and negative control probes for either manual assays or automated assays.