BaseScope Assays

Visualize a specific RNA locus at single cell sensitivity with spatial and morphological context

The BaseScope Assay is a unique product from ACD, based on the same platform of proven and established RNAscope™ technology that has been cited in over 8,000 publications in over 10 years. This powerful assay provides new data dimensions and unique insight into biological mechanisms. The BaseScope Assay enables applications such as the detection of exon junctions/splice variants, short/highly homologous RNA sequences (50-300 bases), and point mutations at single cell sensitivity.

• Detecting biological events in cells and in situ using a single Z pair
• Highly specific and sensitive detection of RNA targets with down to ONE nucleotide differences
• Analysis of broad sample types: FFPE cells & tissues (TMAs), Fresh or fixed frozen tissues, Cultured cells

Exon Junctions / Splice Variants	Short Sequences	Point Mutations
	Gene therapy/AAV
Exon Junctions / splice variants	Short / highly homologous gene sequences (50-300nt)	Point Mutations (ACD validated point mutations only)
Circular RNA (circRNA)	T cell receptors (TCRs) and CDR sequences in T-cell clones	Short InDel
Gene fusion	Gene editing / CRISPR	Homologous
Gene knockout (KO)	Pre-miRNA
	Microexons
Splice Variant Example: Detection of EGFRvIII+ in glioblastoma with the BaseScope v2 Assay	Gene Editing Example: Discern cell type-specific gene editing with the BaseScope Duplex Assay	Point Mutation Example: Detection of KRAS G12D in KRAS mutation cell line with the BaseScope v2 Assay