What is the Probe Name Nomenclature?
ACD Target Probe Name Nomenclature
- ProbeProbe Type
- C2Amplification Channel
These are three types of RNAscope probes. The type of probe is indicated as follows:
for Manual Assays
for Leica Systems automated assays
for Ventana Systems automated assays
Species refers to the probe target species and implies that the probe is designed to the gene of the specified species. The species is typically identified by two letter designations such as "Hs" for Homo Sapiens, "Mm" for Mus musculus etc
This refers to the Official Gene Symbol as listed in the NCBI Database
This indicates any specific target region within the gene
This indicates the probe amplification channel denoted by C1, C2 or C3. RNAscope probes default to Channel 1 unless specified. This channel indicates the compatibility of the probes with different amplification systems and indicate which detection kits the probes would be compatible with.
C1 probes are compatible with all detection platforms
C2 probes are compatible only with the 2.5 HD Duplex Detection Kit and the Fluorescent Detection Kit
C3 channel probes are only compatible with the Fluorescent Detection Kit
This probe used in Leica Automated System Assay(LS Probe) and is designed to hybridize to Mus musculus (Mm) Rspo3 gene. Its spectral channel is not listed which means Spectral Channel 1 and therefore compatible with any of the four manual asays including RNAscope 2.0 HD Reagent Kit-BROWN, RNAscope 2.0 HD Reagent Kit-RED, RNAscope 2-Plex Reagent Kit or RNAscope Multiplex Fluorescent Reagent Kit.
This is probe is designed to be used with a manual assay (Probe). It targets just the 5' untranslated region (5UTR) of the GNRHR gene from the human sequence(Hs). This probe is compatible with spectral channel 2 (C2), so it can be used in a duplex or multiplex assay.