Fresh frozen tissue

miRNAscope™ HD Reagent Kit ? RED Assay is based on ACD's patented signal amplification and background suppression technology. This is a highly sensitive RNA ISH technique and can be used for small RNAs including ASOs, miRNAs, and siRNAs. The miRNAscope™ HD Reagent Kit ? RED assay includes the Fast Red dye which offers a higher contrast and is the first choice in situ hybridization application where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens.

miRNAscope™ HD Detection Reagents - RED assay is designed for RNA in situ hybridization (ISH) based on ACD's patented signal amplification and background suppression technology. This manual assay is a single-plex, chromogenic-RED assay. The detection reagents contains AMP 1, APM2, AMP 3, AMP 4, AMP 5, AMP 6, Fast RED-A, Fast RED-B. The detection reagents provide enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in).To run the detection reagents you will need the pretreatment kit and the wash buffer reagents.

RNAscope pretreatment reagents for sample permealization using Hydrogen peroxide, Protease Plus, Protease III, and Protease IV. It contains: Two bottles each of Hydrogen peroxide, Protease Plus, Protease III, and Protease IV reagents. Note this reagent kit can be used for Fresh Frozen and Adherent or Cultured Cells which do not require target retrieval steps. Disclaimer product photos will be updated shortly. This kit is part of the Universal Pretreatment Kit.

RNAscope™ Intro Pack for Multiplex Fluorescent Reagent Kit v2- Mm recommended for new users getting started with RNAscope assay on mouse samples. It includes required materials such as: RNAscope™ Multiplex Fluorescent Reagents Kit ,FFPE Control Slide Pack -Mouse 3T3 Cell Pellet (PN 310023),Hydrophobic Barrier Pen (PN 310018), RNAscope™ 3-plex Positive Control Probe_Mm(PN 320881), RNAscope™ 3-plex Negative Control Probe(PN 320871) and RNAscope™ Probe Diluent (300041). Users can use the control slides and the probes, to get familiar with the assay workflow and staining results.

Universal Pretreatment Reagents is a pretreatment reagent bundle for users likely to switch across different tissue types. It contains hydrogen peroxide (2 bottles) , target retrieval (4 bottles), Protease Plus (2 bottles), Protease III (2 bottles) and Protease IV (2 bottles). With these reagents, prepare your samples for RNAscope manual assay with any of the following - FFPE, Fresh frozen or Fixed frozen tissues and or Adherent or Cultured Cells. Note:Compatible with RNAscope and BaseScope Amplification reagents.

RNAscope™ reagents used to pretreat fresh frozen tissue samples and allow access to the target RNA. It contains one vial of Protease III and two vials of 2x Protease IV reagents.

RNAscope™ Multiplex Fluorescent Reagent Kit v2 is a RNA in situ hybridization (ISH) assay designed as a fluorescent dye for single plex, duplex and up to 3-plex capability. Each reagent kit provides enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in). Each kit contains: RNAscope Multiplex Fluorescent Detection Kit (PN 323110), RNAscope H202 and Protease Reagents (PN 322381) and RNAscope Target Retrieval reagents (322000), RNAscope Wash Buffer (PN 310091).

RNAscope™ Multiplex Fluorescent Detection Kit v2 is a RNA in situ hybridization (ISH) assay designed as a fluorescent dye for single plex, duplex and up to 3-plex capability. Each detection kit provides enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in). Each kit contains: RNAscope Multiplex Fluorescent Detection Kit (PN 323110). Opal fluorophores from Akoya Biosciences must be purchased separately (Opal 520, PN FP1487001KT; Opal 570, PN FP1488001KT; Opal 690, PN FP1497001KT).

RNAscope™ 4-Plex Ancillary kit is designed to work with RNAscope Multiplex Flourescent Reagent Kit v2 (323100) for 4 plex fluorescent in situ hybridization. It contains C4 HRP and blocker. Note: Opal fluorophores from Akoya Biosciences must be purchased separately (Opal 520, PN FP1487001KT; Opal 570, PN FP1488001KT; Opal 620, PN FP1495001KT; Opal 690, PN FP1497001KT). Multi spectral imaging systems are required for adequate imaging of 4 plex fluorescent images

The RNAscope™ 2.5 High Definition(HD)- RED Assay, is based on ACD's patented signal amplification and background suppression technology. The 2.5 HD version is a high sensitive RNA ISH technique and can be used for low expressing gene targets. The RNAscope™ 2.5 HD Reagent Kit-RED assay includes the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens.