Pretreatment reagents are used for blocking endogenous peroxidase activity and to optimally permeabilize properly prepared samples. These reagents have been optimized to better access target RNA and reduce optimization time in multiple tissues such as formalin-fixed, paraffin-embedded (FFPE) (including archival) tissue, fresh‑frozen (FF) tissue, fixed‑frozen tissue, tissue microarray (TMA), and cell preparations. 

The pretreatment reagents listed in this section are for the manual RNAscope® and BaseScope™ Assays. Please refer to the appropriate sample preparation guides as shown in the User Manual Selection Guide available here to ensure your samples are prepared correctly for the RNAscope® and BaseScope™ detection assay.
RNAscope® 2.5 Universal Pretreatment Reagents (322380): this kit includes all the pretreatment reagents that may be used across different tissue types:
- RNAscope® Target Retrieval Reagent
- RNAscope® Hydrogen Peroxide Reagent
- RNAscope® Protease III Reagent*
- RNAscope® Protease IV Reagent*
- RNAscope® Protease Plus Reagent*

The BaseScope™ Reagent kit is shipped with the Universal Pretreatment kit. 
 

Select the pretreatment reagents you need based on the sample type and detection assay type:

Tissue TypeDetection Assay TypeReagent to use for pretreatment
FFPE (Formalin-Fixed Paraffin-Embedded )RNAscope® 2.5 HD Brown, Red, DuplexRNAscope® Hydrogen Peroxide
RNAscope® Target Retrieval
RNAscope® Protease Plus*
RNAscope® Multiplex Fluorescent v2RNAscope® Hydrogen Peroxide
RNAscope® Target Retrieval
RNAscope® Protease III
BaseScope™ RedRNAscope® Hydrogen Peroxide
RNAscope® Target Retrieval
RNAscope® Protease III
Fixed FrozenRNAscope® 2.5 HD Brown, Red, DuplexRNAscope® Hydrogen Peroxide
RNAscope® Target Retrieval
RNAscope® Protease Plus
RNAscope® Fluorescent MultiplexRNAscope® Target Retrieval
RNAscope® Protease III
RNAscope® Multiplex Fluorescent v2RNAscope® Hydrogen Peroxide
RNAscope® Target Retrieval
RNAscope® Protease III
Fresh frozenRNAscope® 2.5 HD Brown, Red, DuplexRNAscope® Hydrogen Peroxide
RNAscope® Protease IV*
RNAscope® Fluorescent MultiplexRNAscope® Protease IV
RNAscope® Multiplex Fluorescent v2RNAscope® Hydrogen Peroxide
RNAscope® Protease IV
BaseScope™ RedRNAscope® Hydrogen Peroxide
RNAscope® Protease IV
Cultured Adherent cellsRNAscope® 2.5 HD Brown, Red, DuplexRNAscope® Hydrogen Peroxide
RNAscope® Protease III
RNAscope® Fluorescent MultiplexRNAscope® Protease III
PBMC (Peripheral Blood Mononuclear Cells)/Non-Adherent CellsRNAscope® Fluorescent MultiplexRNAscope® Protease III

*The protease concentration across RNAscope® Protease Plus, Protease III and Protease IV is: Protease IV (strong conc.) > Protease III (standard)> Protease Plus (mild)

Product Ordering Information

322000 RNAscope® Target Retrieval Reagents
322340 RNAscope® Protease III & Protease IV Reagents
322330 RNAscope® H202 & Protease Plus Reagents
322380 RNAscope® 2.5 Universal Pretreatment Reagents.

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