The BaseScope™ Reagent Kit v2-RED, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. The BaseScope™ Assay is used for the detection of short RNA targets (50-300 bases), highly homologous sequences, RNA mutations, and exon junctions for the detection of splice variants, circular RNA, and gene fusion. The BaseScope™ Assays do not require an RNase-free environment that is used for traditional RNA ISH.
Applications of the BaseScope™ RED Assay
| Exon Junctions/ Splice Variants | Short/Homologous Target Sequences | Point Mutations* |
|---|---|---|
| Exon junctions/splice variants | Short targets/highly homologous gene sequences (50-300nt) | Point mutation |
| Circular RNA (cirRNA) | T cell receptors (TCRs) and CDR sequences in T-cell clones | Short InDel |
| Gene fusion | pre-miRNA | Homologues |
| Gene knockout (KO) | Gene editing/CRISPR | * ACD Validated Point Mutations Only |
One major application for the BaseScope™ RED Assay is to identify highly homologous short target sequences. The BaseScope™ RED Assay can discern highly homologous MAGEA gene family in intact tissue with morphological context.
BaseScope™ Probes for MAGEA Gene Family Detection
The BaseScope™ Reagent Kit v2-RED with Fast Red dye offers high contrast and easy dot visualization.
The BaseScope™ Assay Workflow Requires:
BaseScope™ Target Probes (Catalog or Made-to-Order C1 Probes)
BaseScope® Control Probes (Select positive control probes and negative control probes)
Control Slides (optional)
BaseScope™ Reagent Kit v2-Red (Universal Pretreatment Kit, Detection kit, and Wash Buffer included)
Brightfield microscope or multispectral fluorescent imaging system
HybEZ™ I or II Hybridization System
