RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH, with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS. Our recent advances in RNAscope® technology include the development of the BaseScope™ assay, which enables detection of any mRNA, short RNA targets and exon junctions. We will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.
- New to RNAscope® manual assay
- New to RNAscope® ISH with IHC experience
- New to RNAscope® ISH with traditional ISH experience
- New to fluorescence ISH with chromogenic ISH experience
- How RNAscope® and BaseScope™ assays work
- What tools are needed for getting started
- Tips and tricks for sample preparation
- FAQs & Troubleshooting tips
- Where to find information
