Using single cell RNAseq and in situ hybridization to determine neuronal composition
Dr. Martin Häring
Postdoc, Institute of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm
Dr Courtney Anderson
Applications Leader, Senior Scientist, Advanced Cell Diagnostics
Outline:

Being aware of and discriminating between different types of cues through the somatosensory system is a critical function for interpreting the internal and external world and for protection from tissue damage. Upon activation by a diverse set of chemical, thermal or mechanical stimuli, primary sensory neurons in the dorsal root ganglion relay this information through their central terminations onto neurons located in the dorsal horn of the spinal cord. The dorsal horn of the spinal cord plays a critical role for discriminating different stimuli as well as processing distinct modalities of innocuous and noxious sensation.

Using single-cell RNA sequencing (scRNA seq) to classify sensory neurons in the mouse dorsal horn and DRG allowed the detection and classification of the respective neuron populations. This essential knowledge will function as a foundation for all future investigations as it allows the question of how a particular sensory stimulus is processed on a cellular level to be addressed.

 

Unable to join the live event? Watch on-demand here.

Learning Objectives:

During this webcast you will:

  • Discover more on the neuronal anatomy of somatosensation
  • Gain insight on how to approach and address a single cell project
  • Get advice on single cell dissociation and RNAscope® techniques
  • Speak to the presenters in a live Q&A session following the presentation.
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