Tips, Tricks & Troubleshooting RNAscope® and BaseScope™ Assays
Marie Lauigan
Technical Support Scientist
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Outline:

RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH, with its proprietary probe design to amplify target-specific signals. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

Our recent advances in RNAscope® technology include the development of the BaseScope™ assay, which enables detection of short RNA targets and exon junctions for the analysis of splice variants, circular RNA and gene fusions.

We will discuss the workflow of both RNAscope® and BaseScope™ assays and share troubleshooting tips and tricks to obtain publication-quality RNA in situ results.

Who should attend:

This webinar will benefit:

  • New and experienced RNAscope® & BaseScope™ technology users.
  • Users performing ISH combined with IHC
  • Traditional ISH users currently performing RNAscope® ISH
Learning Objectives:
  • How RNAscope® and BaseScope™ assays work
  • Workflow of RNAscope® and BaseScope™ assays
  • What tools are needed for getting started
  • Tips and tricks for sample preparation & pretreatment
  • FAQs & Troubleshooting tips
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