RNAscope™ is a proprietary RNA in situ hybridization (ISH) assay based on ACD patented signal amplification and background suppression technology which advances RNA biomarker analysis in tissues and cells. Unique to this technology, RNAscope™ delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. This enables researchers to visualize which genes are expressed, localize where they are expressed, and quantify the level of expression.

Key benefits

• Detection of a single RNA molecule requires only three double Z probe pairs to bind to the target RNA molecule. The RNAscope™ 20 double Z probe pairs design provides robustness against partial target RNA accessibility or degradation.
• Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples. Rigorous double Z probes design eliminates cross hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology.
• Provides cell-specific expression information in intact tissue architecture.
• Works for virtually ANY gene from ANY species in ANY tissue.

Presenters

Morgane Rouault, PhD 

Senior Application and Support Scientist, Europe, Advanced Cell Diagnostics 

“Visualize Gene Expression and Genetic Variations in Tissues”

Yvan Vachez, PhD

"Implication of D2/D3 dopaminergic receptors in neuropsychiatric symptoms of Parkinson's Disease”

Alessia Perino, PhD

“Detecting low expressed one exon genes with RNA scope: the story of the bile acid receptor TGR5” 

Ossama Khalaf, PhD

“Identification of neuronal subpopulation for remote fear memory extinction in the hippocampus”