Testimonial

"Our laboratory has been working with RNAscope for several years. We have been using RNAscope for the detection of EBV traces in lymphomas and epithelial tumours, classified as negative by conventional techniques. Thanks to the sensitivity of RNAscope, we have been able to obtain very good results. Recently we were able to automate EBER and EBNA1 RNAscope probes on the Leica BOND RX system, and thanks to the ACD support team, we optimized the protocols and achieved results in a short time." 

“My research is focused on looking at microglia during postnatal development in the hypothalamus to understand how they're interacting with AgRP neurons and facilitating establishment of neural circuits that control food intake. RNAscope will be a huge part in helping us understand the molecular mechanisms that may contribute to how microglia are interacting with the AgRP terminals.”

“I honestly can't rave about RNAscope enough. We work in ferrets and it's very unique, so we have to use all custom probes. So far all of the probes we've used, even though they're custom made and nobody has used them before, have all been hits. I tell everyone that I love RNAscope over immunoassays, or Westerns, or anything like that.”

"The identification and anatomic location of HIV-infected cells and the effects of tissue environment on virus replication and viral gene expression are all critical for evaluating the pathogenesis of HIV infection and for developing cure strategies to eliminate HIV reservoirs. RNAscope in situ hybridization (ISH) has enabled us to visualize and quantify HIV RNA with single-copy resolution.

We used ACD’s RNAscope™ in situ hybridization assay service for co-localization analysis and gene expression profiling of several RNA biomarkers. For our project, we chose to use both the RNAscope chromogenic duplex and RNAscope fluorescent duplex assays. We are very pleased with the high resolution and quality of the raw data along with their expertise and timely delivery of the report and materials. The technology is impressive and is coupled with friendly service. A positive experience overall, we would highly recommend them for ISH assay development and implementation.

RNAscope has streamlined in situ RNA hybridization to the point were it can become a standard technique easily implemented in most labs, even by individuals with no experience with this often difficult technique. Moreover the ability to multiplex ISH to analyze simultaneously mRNA levels of up to three different genes allowed the study of changes in gene expression in specific cell populations in the CNS in response to different experimental conditions.

RNAscope SpotStudio Software is very easy to use. I especially like the ability to interact with the image in the result section—to connect the quantitative result to the image of the cell in question is invaluable.

…I had good experience with the RNAscope™ in situ hybridization assay from Advanced Cell Diagnostics. Even though I had no prior experience with RNA in situ hybridization, RNAscope assay was fairly easy to perform and it worked for me on the first try. Of course, as I used the assay more, a few adjustments were done. I tried both the chromogenic 2.0 and multiplex fluorescent assays, and they worked well for me. I would highly recommend buying ACD’s HybEZ Oven which makes performing the assay much more convenient.

We’ve had a great experience working with ACD. Using their service team, we are able to move faster through our testing for Phase I trial, we are very happy with the quality of data, thoroughness in the reports we receive and would  highly recommend them for ISH assay development and implementation.

We are studying molecular mechanisms leading to neural developmental disorders using mouse, non-human primates and human tissues. To gain further insight into up-or downstream effects for specific type molecules in neurons/glia, co-localization and cell type specific gene expression analyses are invaluable. ACD’s RNAscope in situ hybridization is a powerful tool for this purpose. In our projects, we mainly use multiplex fluorescent assays combined with confocal microscopy on sectioned mouse brains.