Publication

本研究结合教学实践，对病理学专业学位研究生病理技术教学内容、方式及效果评价进 行总结。根据研究生基础条件制定病理技术培训方案，采用翻转课堂等多元化教学方法开展以学生 为中心的启发式教学，利用智能化教学平台开展互动式理论教学，以问题导向学习模式开展实践教 学，旨在提高病理学专业学位研究生的病理技术理论素养和实践水平，提升临床科研思维，为其将来 开展临床病理诊断和科研工作奠定基础。

马钱子中可以提取出士的宁、马钱子碱、环烯醚萜苷等多种具有生物活性的化合物。 综述了马钱子主要提取物在神经系统、免疫系统、肌肉骨骼系统、抑制病原微生物和抗肿瘤 方面的药理机制，并分析了其活性成分的临床应用价值。结果发现马钱子活性成分具有可 兴奋中枢神经系统，提高感觉器官的功能，有效减轻炎症反应，有较强的镇痛抗炎功能，此外 还对多种肿瘤具有抑制效果，未来有望开发成为抗血管增生和抗癌治疗药物。

We aimed to identify the underlying genetic cause for a novel form of distal arthrogryposis.Rare variant family-based genomics, exome sequencing, and disease-specific panel sequencing were used to detect ADAMTS15 variants in affected individuals. Adamts15 expression was analyzed at the single-cell level during murine embryogenesis.

Adult hippocampal neurogenesis (AHN) is a well-studied phenomenon that involves the derivation of new neurons from neural progenitor cells in the dentate gyrus region of the hippocampus, an area responsible for cognitive functions such as learning and memory storage. Moreover, the hippocampus is known to be implicated in neurological conditions such as Alzheimer's disease. Although AHN has been extensively observed in animal models for twenty years, its existence and persistence in humans have been widely debated in academia, heavily based on post-mortem immunohistochemical markers.

The development of multi-omic profiling tools has rapidly expanded in recent years, along with their use in profiling skin tissues in various contexts, including dermatologic diseases. Among these tools, single-cell RNA-sequencing (scRNA-seq) and spatial transcriptomics (ST) have emerged as widely adopted and powerful assays for elucidating key cellular components and their spatial arrangement within skin disease.

Mechanosensation begins with the sensing of pressure by mechanically activated (MA) channels in the nerve endings of dorsal root ganglion (DRG) neurons. Piezo1, a fast-inactivating MA channel, has surfaced to be involved in pruriception. However, the pressure-dependent activation mechanism and its physiological role in mechanical pain remain unidentified. Here, we report that _Piezo1_ is expressed in a small DRG subpopulation, which is largely positive for _TRPV1_ rather than _MRGPRD_, which is known for nociceptors.

Loss of caveolin-1 (Cav-1) and upregulation of monocarboxylate transporters (MCTs, especially MCT1 and MCT4) in respectively tumor-associated stromal cells and malignant epithelial cells of invasive carcinoma have been found to play an important role in the metabolic coupling. However, this phenomenon has only been scarcely described in pure ductal carcinoma in situ (DCIS) of the breast.

Development of spatial-integrative pre-clinical models is needed for glioblastoma, which are heterogenous tumors with poor prognosis. Here, we present an optimized protocol to generate three-dimensional ex vivo explant slice glioma model from orthotopic tumors, genetically engineered mouse models, and fresh patient-derived specimens. We describe a step-by-step workflow for tissue acquisition, dissection, and sectioning of 300-μm tumor slices maintaining cell viability.

A mesiodens is a supernumerary tooth located in the midline of the premaxilla. To investigate the genetic cause of mesiodens, clinical and radiographic examination were performed on 23 family members of a two-generation Hmong family. Whole exome sequencing (WES) or Sanger sequencing were performed in 22 family members and two unrelated Thai patients with mesiodens. WES in the Hmong family revealed a missense mutation (c.1807G>A;p.Glu603Lys) in PTPN23 in seven affected members and six unaffected members. The mode of inheritance was autosomal dominance with incomplete penetrance (53.84%).

Abstract Unavailable