Faqs

No. RNAscope™ 2.5 HD has been optimized with RNAscope™ Hydrogen Peroxide, Target Retrieval Reagents, and Protease Plus, Protease III & Protease IV.

Yes, RNAscope™ 2.0 HD will be in stock for a limited time. Please contact your sales representative for more information.

As of October 14, 2015 we will be ready to ship RNAscope™ 2.5 kits. If you have an RNAscope™ 2.0 HD order in process and would like to switch, contact your ACD account manager. Note: RNAscope™ 2.0 HD will officially be discontinued on June 30, 2016 and support will continue to June 1, 2017.

The product will be ready to ship on October 14, 2015, (in the U.S. & E.U.). Contact your ACD account manager to order. Direct online orders will be available in the U.S. by October 22, 2015.

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The new 2.5 assay will be easier to use and more reproducible resulting in improved product performance. Manual chromogenic Brown/Red kits will require fewer steps for tissue optimization, and detection reagents no longer need to be pre-warmed.

Antigen retrieval conditions often require optimization in both RNAscope and IHC, depending on the tissue type and how the sample was fixed and processed. We recommend optimizing conditions for the RNAscope Assay if tissue samples are not prepared according to the ACD recommended guideline of fixing samples in fresh 10% NBF for 16–32 hours.

The main differences compared to an IHC workflow are the following:

RNAscope Technology is a novel in situ hybridization (ISH) assay that detects target RNA within intact cells. The assay is based on a patented signal amplification and background suppression technology, representing a major advance over traditional RNA ISH. RNAscope Assays do not require an RNase-free environment.
 

Both temperature and humidity are critical to assay performance. The HybEZ™ oven is the only hybridization oven that ACD has extensively tested and validated. Other incubators/hybridization stations may not provide consistent results.

Protease digestion is also critical to assay performance. Under-digestion will result in lower signal and a ubiquitous background. Over-digestion will result in poor morphology and loss of RNA.

You have a few options, when you plan your assay-

C1 probe can be substituted with blank probe diluent and used with C2, C3 or C4 target probes for duplex/multiplex assay. Do not use channel C2 or channel C3 probes with RNAscope 2.5 HD singleplex Brown or 2.5 HD Red assays. These are designed to work only with C1 probes.