RNASCOPE® TECHNOLOGY
June 9, 2017
12 - 1 pm
MRC Weatherall Institute of Molecular Medicine (WIMM)
University of Oxford
John Radcliffe Hospital
Oxford, UK
The RNAscope™ technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.
Matthew Nolan will present on the RNAscope™ application in Neuroscience “In-situ expression of post-mitotic transcription factors associated with projection neuron diversity in the adult post-mortem brain”
In addition Farai Shah will give an overview on:
Visualizing gene expression and genetic variations in tissue environments of Neuroscience and Cancer related research by RNAscope™ and BaseScope™
- Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope™ assay
- Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
- Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
- Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system:
- Co-expression with specific cell type markers
- Sub-cellular localization of mRNA expression in cell body, axons or dendrites
- Distinguish specific homologues, paralogues, and orthologues by RNAscope™ assay or BaseScope™ assay
- Perform single cell analysis with morphological information in FFPE or frozen tissue
- Pinpoint expression of novel RNAs of interest
