RNASCOPE® TECHNOLOGY
March 29, 2017
12.30 to 13.30
Institute of Reproductive and Developmental Biology (IRDB)
Imperial College London
Du Cane Road, London
Ground Floor Seminar Room
The RNAscope™ technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.
The seminar will give an overview on:
Visualizing gene expression and genetic variations in tissue environments by RNAscope™ and BaseScope™
- Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope™ assay
- Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
- Identify point mutations or gene editing by BaseScope™ assay
- Distinguish specific homologues, paralogues, and orthologues by RNAscope™ assay or BaseScope™ assay
- Perform single cell analysis with morphological information in FFPE or frozen tissue
- Pinpoint expression of novel RNAs of interest
Comprehensive evaluation of the tumor-immune environment by RNAscope™ and BaseScope™
- Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
- Carry out quantitative analysis of co-expression of multiple checkpoint molecules within the same cell
- Evaluate immune function by visualizing mRNA expression of critical molecules including IFNg and other cytokines and chemokines in the TME
- Assess the distribution of specific T cell clones, detected by specific CDR sequences, in tumoral and stromal environments
- Go beyond the previous restriction of short sequence limitations
